Three types of Photosystem II photoinactivation - I. Damaging processes on the acceptor side

Ivan Šetlík, Suleyman Allakhverdiev, Ladislav Nedbal, Eva Šetlíková, Vyacheslav V. Klimov

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Oxygen evolving photosystem II particles were exposed to 100 and 250 W m-2 white light at 20°C under aerobic, anaerobic and strongly reducing (presence of dithionite) conditions. Three types of photoinactivation processes with different kinetics could be distinguished: (1) The fast process which occurs under strongly reducing (t1/2≅1-3 min) and anaerobic conditions (t1/2≅4-12 min). (2) The slow process (t1/2≅15-40 min) and (3) the very slow process (t1/2>100 min), both of which occur under all three sets of conditions. The fast process results in a parallel decline of variable fluorescence (Fv) and of Hill reaction rate, accompanied by an antiparallel increase of constant fluorescence (Fo). We assume that trapping of QA in a negatively charged stable state, (QA -)stab, is responsible for the effects observed. The slow process is characterized by a decline of maximal fluorescence (Fm). In presence of oxygen this decline is due to the well known disappearance of Fv which proceeds in parallel with the inhibition of the Hill reaction; Fo remains essentially constant. Under anaerobic and reducing conditions the decline of Fm represents the disappearance of the increment in Fo generated by the fast process. We assume that the slow process consists in neutralization of the negative charge in the domain of QA in a manner that renders QA non-functional. The charge separation in the RC is still possible, but energy of excitation becomes thermally dissipated. The very slow photoinactivation process is linked to loss of charge separation ability of the PS II RC and will be analyzed in a forthcoming paper.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalPhotosynthesis Research
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
photosystem II
Fluorescence
fluorescence
Oxygen
Dithionite
oxygen
white light
neutralization
anaerobic conditions
Reaction rates
trapping
Light
kinetics
Kinetics
energy

Keywords

  • chlorophyll a fluorescence
  • oxygen evolving PS II particles
  • pheophytin
  • photoinhibition
  • photosystem II
  • primary electron acceptor Q

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Šetlík, I., Allakhverdiev, S., Nedbal, L., Šetlíková, E., & Klimov, V. V. (1990). Three types of Photosystem II photoinactivation - I. Damaging processes on the acceptor side. Photosynthesis Research, 23(1), 39-48. https://doi.org/10.1007/BF00030061

Three types of Photosystem II photoinactivation - I. Damaging processes on the acceptor side. / Šetlík, Ivan; Allakhverdiev, Suleyman; Nedbal, Ladislav; Šetlíková, Eva; Klimov, Vyacheslav V.

In: Photosynthesis Research, Vol. 23, No. 1, 01.01.1990, p. 39-48.

Research output: Contribution to journalArticle

Šetlík, I, Allakhverdiev, S, Nedbal, L, Šetlíková, E & Klimov, VV 1990, 'Three types of Photosystem II photoinactivation - I. Damaging processes on the acceptor side', Photosynthesis Research, vol. 23, no. 1, pp. 39-48. https://doi.org/10.1007/BF00030061
Šetlík, Ivan ; Allakhverdiev, Suleyman ; Nedbal, Ladislav ; Šetlíková, Eva ; Klimov, Vyacheslav V. / Three types of Photosystem II photoinactivation - I. Damaging processes on the acceptor side. In: Photosynthesis Research. 1990 ; Vol. 23, No. 1. pp. 39-48.
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