Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae

Shuhei Ohkita, Yui Lee, Quyet Nguyen, Kenichi Ikeda, Nobuhiro Suzuki, Hitoshi Nakayashiki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

Original languageEnglish
Pages (from-to)25-35
Number of pages11
JournalVirology
Volume534
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

RNA
Viruses
Gene Silencing
Open Reading Frames
Triticum
Genome
Oryza

Keywords

  • Mycovirus
  • Ourmia-like virus
  • Subviral RNA

ASJC Scopus subject areas

  • Virology

Cite this

Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae. / Ohkita, Shuhei; Lee, Yui; Nguyen, Quyet; Ikeda, Kenichi; Suzuki, Nobuhiro; Nakayashiki, Hitoshi.

In: Virology, Vol. 534, 01.08.2019, p. 25-35.

Research output: Contribution to journalArticle

Ohkita, Shuhei ; Lee, Yui ; Nguyen, Quyet ; Ikeda, Kenichi ; Suzuki, Nobuhiro ; Nakayashiki, Hitoshi. / Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae. In: Virology. 2019 ; Vol. 534. pp. 25-35.
@article{f835f788b8c94a55a24a0cf64190ea65,
title = "Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae",
abstract = "Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.",
keywords = "Mycovirus, Ourmia-like virus, Subviral RNA",
author = "Shuhei Ohkita and Yui Lee and Quyet Nguyen and Kenichi Ikeda and Nobuhiro Suzuki and Hitoshi Nakayashiki",
year = "2019",
month = "8",
day = "1",
doi = "10.1016/j.virol.2019.05.015",
language = "English",
volume = "534",
pages = "25--35",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Three ourmia-like viruses and their associated RNAs in Pyricularia oryzae

AU - Ohkita, Shuhei

AU - Lee, Yui

AU - Nguyen, Quyet

AU - Ikeda, Kenichi

AU - Suzuki, Nobuhiro

AU - Nakayashiki, Hitoshi

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

AB - Three ourmia-like viruses, designated Pyricularia oryzae ourmia-like virus (PoOLV) 1 to 3, were identified in a wheat-infecting isolate of P. oryzae. The sizes of the full-length PoOLV1-3 genomes were determined to be 2,528, 1,671, and 2,557 nt. Interestingly, we also found two abundant single-stranded RNAs sharing their 5’ terminal 25 and 255 nt with PoOLV1 RNA and PoOLV3 RNA, respectively. The PoOLV1- and PoOLV3-associated RNAs (ARNA1 and ARNA3) were 639 and 514 nt in length, and possessed one and two small ORFs, respectively. In the field isolates of P. oryzae, PoOLVs and ARNAs were detectable at varying levels, and the levels of PoOLV1 and ARNA1 as well as those of PoOLV3 and ARNA3, were tightly correlated. In addition, gene silencing of PoOLV1 and PoOLV3 resulted in a reduction of ARNA1 and ARNA3, respectively. There results indicated that replication of ARNA1 and ARNA3 was associated with PoOLV1 and PoOLV3, respectively.

KW - Mycovirus

KW - Ourmia-like virus

KW - Subviral RNA

UR - http://www.scopus.com/inward/record.url?scp=85067056677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85067056677&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2019.05.015

DO - 10.1016/j.virol.2019.05.015

M3 - Article

VL - 534

SP - 25

EP - 35

JO - Virology

JF - Virology

SN - 0042-6822

ER -