TY - JOUR
T1 - Three-dimensional visualization of multiple synapses in thick sections using high-voltage electron microscopy in the rat spinal cord
AU - Satoh, Keita
AU - Takanami, Keiko
AU - Murata, Kazuyoshi
AU - Kawata, Mitsuhiro
AU - Sakamoto, Tatsuya
AU - Sakamoto, Hirotaka
N1 - Funding Information:
This work was supported in part by KAKENHI from the Ministry of Education, Science, Sports, Culture and Technology (MEXT), Japan (to K.T.; 26870496 and H.S.; 24680039, 15K15202 and 15H05724 ), by the Naito Memorial Grant for Natural Science Researches, Japan (to K.T. and H.S.), by the Kato Memorial Bioscience Foundation, Japan (to H.S.), by the Life Science Foundation of Japan (to H.S.), and by the Co-operative Study by HVEM ( H-1250M ) of the National Institute for Physiological Sciences, Okazaki, Japan (to K.S., K.T., M.K. and H.S.).
Publisher Copyright:
© 2015 The Authors.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - This data article contains complementary figure and movies (Supplementary Movies 1-3) related to the research article entitled, "Effective synaptome analysis of itch-mediating neurons in the spinal cord: a novel immunohistochemical methodology using high-voltage electron microscopy" [7]. It is important to show the synaptic connections at the ultrastructural level to understand the neural circuit, which requires the three-dimensional (3-D) analyses in the electron microscopy. Here, we applied a new sample preparation method, a high-contrast en bloc staining according to the protocol of the National Center for Microscopy and Imaging Research (NCMIR), University of California, San Diego, CA, USA to high-voltage electron microscopy (HVEM) tomography in order to examine the 3-D chemical neuroanatomy of the rat spinal cord. Pre-embedding immunoelectron microscopy was used in this study. HVEM has an excellent potential to directly visualize the ultrastructures in semi-thin sections (~5. μm thick), and we have successfully visualized many itch-mediating synaptic connections and neural networks in the spinal cord using "HVEM tomography". Moreover, the methodology used in this study is simple and can be applied in multiple ways. This is an important contribution to ultrastructural investigations of the central nervous system in the present post-genomic age.
AB - This data article contains complementary figure and movies (Supplementary Movies 1-3) related to the research article entitled, "Effective synaptome analysis of itch-mediating neurons in the spinal cord: a novel immunohistochemical methodology using high-voltage electron microscopy" [7]. It is important to show the synaptic connections at the ultrastructural level to understand the neural circuit, which requires the three-dimensional (3-D) analyses in the electron microscopy. Here, we applied a new sample preparation method, a high-contrast en bloc staining according to the protocol of the National Center for Microscopy and Imaging Research (NCMIR), University of California, San Diego, CA, USA to high-voltage electron microscopy (HVEM) tomography in order to examine the 3-D chemical neuroanatomy of the rat spinal cord. Pre-embedding immunoelectron microscopy was used in this study. HVEM has an excellent potential to directly visualize the ultrastructures in semi-thin sections (~5. μm thick), and we have successfully visualized many itch-mediating synaptic connections and neural networks in the spinal cord using "HVEM tomography". Moreover, the methodology used in this study is simple and can be applied in multiple ways. This is an important contribution to ultrastructural investigations of the central nervous system in the present post-genomic age.
UR - http://www.scopus.com/inward/record.url?scp=84939180688&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939180688&partnerID=8YFLogxK
U2 - 10.1016/j.dib.2015.07.005
DO - 10.1016/j.dib.2015.07.005
M3 - Article
AN - SCOPUS:84939180688
VL - 4
SP - 566
EP - 570
JO - Data in Brief
JF - Data in Brief
SN - 2352-3409
ER -