Three-dimensional ultrastructural changes of acellular glomerular basement membrane in various types of human glomerulonephritis

S. Nishimura, Hirofumi Makino, Z. Ota

Research output: Contribution to journalArticle

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Abstract

Using a cellular digestion technique combined with scanning electron microscopy, we examined the three-dimensional ultrastructural changes of the glomerular basement membrane (GBM) in various types of human glomerulonephritis (GN). Gaps of the GBM, which were not found by routine transmission electron microscopy, were observed in a patient with IgA nephropathy manifesting microscopic hematuria. The dense material of the GBM in dense deposit disease (DDD) was not affected by this cellular digestion technique, while the deposits in other immune complex diseases were almost removed. Thus the dense material in DDD differs in composition from the immune complex deposits in other GN. In membranous GN and lupus nephritis, the morphological changes of the urinary surface of the GBM varied from pinhole to craters and reticula, according to the severity of the disease stage. This technique is useful for examining ultrastructural changes of the GBM in various human GN.

Original languageEnglish
Pages (from-to)9-17
Number of pages9
JournalNephron
Volume53
Issue number1
Publication statusPublished - Jan 1 1989

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Glomerular Basement Membrane
Glomerulonephritis
Membranoproliferative Glomerulonephritis
Digestion
Immune Complex Diseases
Membranous Glomerulonephritis
Reticulum
Lupus Nephritis
Hematuria
Antigen-Antibody Complex
Transmission Electron Microscopy
Electron Scanning Microscopy
Immunoglobulin A

ASJC Scopus subject areas

  • Nephrology

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Three-dimensional ultrastructural changes of acellular glomerular basement membrane in various types of human glomerulonephritis. / Nishimura, S.; Makino, Hirofumi; Ota, Z.

In: Nephron, Vol. 53, No. 1, 01.01.1989, p. 9-17.

Research output: Contribution to journalArticle

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