Three-dimensional observation and analysis of remineralization in dentinal caries lesions

Kumiko Yoshihara; Noriyuki Nagaoka; Akiko Nakamura; Toru Hara; Satoshi Hayakawa; Yasuhiro Yoshida; Bart Van Meerbeek

doi:10.1038/s41598-020-61111-1

Three-dimensional observation and analysis of remineralization in dentinal caries lesions

Kumiko Yoshihara, Noriyuki Nagaoka, Akiko Nakamura, Toru Hara, Satoshi Hayakawa, Yasuhiro Yoshida, Bart Van Meerbeek

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 μm, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.

Original language	English
Article number	4387
Journal	Scientific reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-61111-1
Publication status	Published - Dec 1 2020

ASJC Scopus subject areas

General

Access to Document

10.1038/s41598-020-61111-1

Cite this

@article{e0a7a0dc76654bf8b518112e0a2abff0,

title = "Three-dimensional observation and analysis of remineralization in dentinal caries lesions",

abstract = "The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 μm, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.",

author = "Kumiko Yoshihara and Noriyuki Nagaoka and Akiko Nakamura and Toru Hara and Satoshi Hayakawa and Yasuhiro Yoshida and {Van Meerbeek}, Bart",

note = "Funding Information: This work was supported by the NIMS microstructural characterization platform as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) in Japan. This study was supported by JSPS KAKENHI [grant number JP 16K2045508, 18K17068]. Publisher Copyright: {\textcopyright} 2020, The Author(s).",

year = "2020",

month = dec,

day = "1",

doi = "10.1038/s41598-020-61111-1",

language = "English",

volume = "10",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Three-dimensional observation and analysis of remineralization in dentinal caries lesions

AU - Yoshihara, Kumiko

AU - Nagaoka, Noriyuki

AU - Nakamura, Akiko

AU - Hara, Toru

AU - Hayakawa, Satoshi

AU - Yoshida, Yasuhiro

AU - Van Meerbeek, Bart

N1 - Funding Information: This work was supported by the NIMS microstructural characterization platform as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) in Japan. This study was supported by JSPS KAKENHI [grant number JP 16K2045508, 18K17068]. Publisher Copyright: © 2020, The Author(s).

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 μm, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.

AB - The remineralization mechanism in dental caries lesions is not completely understood. This study reports on ultrastructural and chemical changes observed within arrested caries lesions. Carious human teeth were observed using scanning electron microscopy (SEM) and focused-ion-beam (FIB)-SEM. The crystals detected in the caries lesions were characterized by transmission electron microscopy (TEM), along with chemical element mapping using energy-dispersive spectroscopy (EDS)-STEM. FIB-SEM 3D reconstructions revealed a severely damaged dentin surface abundantly covered by bacteria. Although the dentin tubules were clogged up to a depth of 100 μm, bacterial invasion into dentin tubules was not observed. TEM crystal analysis and EDS-STEM revealed the presence of Ca and P, as well as of Mg within the HAp crystals deposited inside the dentin tubules. It was concluded that extensive remineralization with deposition of Mg-HAp crystals had occurred in dentin tubules of caries-arrested dentin. Understanding the natural remineralization process is thought to be helpful for developing clinical biomimetic remineralization protocols.

UR - http://www.scopus.com/inward/record.url?scp=85081543345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85081543345&partnerID=8YFLogxK

U2 - 10.1038/s41598-020-61111-1

DO - 10.1038/s41598-020-61111-1

M3 - Article

C2 - 32152430

AN - SCOPUS:85081543345

SN - 2045-2322

VL - 10

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 4387

ER -

Three-dimensional observation and analysis of remineralization in dentinal caries lesions

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this