TY - JOUR
T1 - Three-dimensional morphometry of collagen fibrils in membranous bone
AU - Hashimoto, Mana
AU - Nagaoka, Noriyuki
AU - Tabata, Kaori
AU - Tanaka, Tomoyo
AU - Osumi, Ryuta
AU - Odagaki, Naoya
AU - Hara, Toru
AU - Kamioka, Hiroshi
N1 - Funding Information:
This study was supported by the NIMS microstructural characterization platform as a program of the Nanotechnology Platform of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan, and the Japan Society for the Promotion of Science in the form of Grants-in-Aid for Research (no. JP16H05549 and JP16K15837). The authors would like to thank Akiko Nakamura (NIMS), Yuka Hara (NIMS), and Itsuro Kamimura (Maxnet Co., Ltd).
Publisher Copyright:
© 2017 The Royal Society of Chemistry.
PY - 2017/11
Y1 - 2017/11
N2 - The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.
AB - The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.
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U2 - 10.1039/c7ib00073a
DO - 10.1039/c7ib00073a
M3 - Article
C2 - 29091092
AN - SCOPUS:85034040331
VL - 9
SP - 868
EP - 875
JO - Integrative Biology (United Kingdom)
JF - Integrative Biology (United Kingdom)
SN - 1757-9694
IS - 11
ER -