Three-dimensional morphometry of collagen fibrils in membranous bone

Mana Hashimoto, Noriyuki Nagaoka, Kaori Tabata, Tomoyo Tanaka, Ryuta Osumi, Naoya Odagaki, Toru Hara, Hiroshi Kamioka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.

Original languageEnglish
Pages (from-to)868-875
Number of pages8
JournalIntegrative Biology (United Kingdom)
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 1 2017

Fingerprint

Bone
Collagen
Bone and Bones
Osteoblasts
Skull
Software
Osteocytes
Scaffolds
Organelles
Pixels
Tissue
Scanning electron microscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Three-dimensional morphometry of collagen fibrils in membranous bone. / Hashimoto, Mana; Nagaoka, Noriyuki; Tabata, Kaori; Tanaka, Tomoyo; Osumi, Ryuta; Odagaki, Naoya; Hara, Toru; Kamioka, Hiroshi.

In: Integrative Biology (United Kingdom), Vol. 9, No. 11, 01.11.2017, p. 868-875.

Research output: Contribution to journalArticle

Hashimoto, Mana ; Nagaoka, Noriyuki ; Tabata, Kaori ; Tanaka, Tomoyo ; Osumi, Ryuta ; Odagaki, Naoya ; Hara, Toru ; Kamioka, Hiroshi. / Three-dimensional morphometry of collagen fibrils in membranous bone. In: Integrative Biology (United Kingdom). 2017 ; Vol. 9, No. 11. pp. 868-875.
@article{b03ee03cb4de46a19b28559e64257114,
title = "Three-dimensional morphometry of collagen fibrils in membranous bone",
abstract = "The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.",
author = "Mana Hashimoto and Noriyuki Nagaoka and Kaori Tabata and Tomoyo Tanaka and Ryuta Osumi and Naoya Odagaki and Toru Hara and Hiroshi Kamioka",
year = "2017",
month = "11",
day = "1",
doi = "10.1039/c7ib00073a",
language = "English",
volume = "9",
pages = "868--875",
journal = "Integrative Biology (United Kingdom)",
issn = "1757-9694",
publisher = "Royal Society of Chemistry",
number = "11",

}

TY - JOUR

T1 - Three-dimensional morphometry of collagen fibrils in membranous bone

AU - Hashimoto, Mana

AU - Nagaoka, Noriyuki

AU - Tabata, Kaori

AU - Tanaka, Tomoyo

AU - Osumi, Ryuta

AU - Odagaki, Naoya

AU - Hara, Toru

AU - Kamioka, Hiroshi

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.

AB - The collagen network acts as a scaffold for calcification and its three-dimensional structure influences bone strength. It is therefore important to observe the collagen network in detail and three-dimensionally. In this study, we observed the collagen network of chick embryonic calvariae in membranous bone three-dimensionally using orthogonally arranged FIB-SEM. A 25 × 25 μm area of chick embryonic calvaria was observed at a high resolution (25 nm per pixel). The inside of the bone (i.e. the primary calcified tissue), the bone cells (i.e. the osteoblasts and the osteocytes), the organelles, and the collagen fibrils were observed in detail. These structures were observed three-dimensionally using the Amira software program. In addition, the collagen fibrils of the bone were automatically extracted using the XTracing extension software program, and three-dimensional morphometry was performed. Almost all of the collagen fibrils ran along the longitudinal axis of the trabecular bone. We found that the regularity of the collagen fibril orientation was less remarkable in the osteoblast layer, which contained numerous osteoblasts. The collagen fibril orientation started to show regularity toward the central bone layer, which contained few bone cells.

UR - http://www.scopus.com/inward/record.url?scp=85034040331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034040331&partnerID=8YFLogxK

U2 - 10.1039/c7ib00073a

DO - 10.1039/c7ib00073a

M3 - Article

C2 - 29091092

AN - SCOPUS:85034040331

VL - 9

SP - 868

EP - 875

JO - Integrative Biology (United Kingdom)

JF - Integrative Biology (United Kingdom)

SN - 1757-9694

IS - 11

ER -