Three-dimensional meshwork structure of glomerular basement membrane revealed by chemical treatment

Kenichi Shikata, Hirofumi Makino, A. Ichiyasu, Z. Ota

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to study the three-dimensional ultrastructure of the glomerular basement membrane (GBM), isolated GBM was treated with elastase, sodium dodecyl sulfate, and 2-mercaptoethanol and examined by transmission electron microscopy using ultrathin sectioning method and stereoscopic view. This treatment revealed clearly three-dimensional meshwork structure of GBM mainly composed of type IV collagen. Numerous regular polygonal small pores were present in the meshwork structure. The average short dimension of the small pores was 4.1 ± 1.8 nm and the average long dimension was 4.8 ± 2.2 nm. Our present results further support the molecular sieve theory of the basement membrane.

Original languageEnglish
Pages (from-to)182-185
Number of pages4
JournalJournal of Electron Microscopy
Volume39
Issue number3
Publication statusPublished - 1990

Fingerprint

Glomerular Basement Membrane
basements
membranes
Collagen Type IV
Mercaptoethanol
Pancreatic Elastase
Transmission Electron Microscopy
porosity
Basement Membrane
Sodium Dodecyl Sulfate
Molecular sieves
Sodium dodecyl sulfate
absorbents
collagens
sodium sulfates
Collagen
Transmission electron microscopy
transmission electron microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Three-dimensional meshwork structure of glomerular basement membrane revealed by chemical treatment. / Shikata, Kenichi; Makino, Hirofumi; Ichiyasu, A.; Ota, Z.

In: Journal of Electron Microscopy, Vol. 39, No. 3, 1990, p. 182-185.

Research output: Contribution to journalArticle

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