Three-dimensional meshwork structure of glomerular basement membrane revealed by chemical treatment

Kenichi Shikata, Hirofumi Makino, Akira Ichiyasu, Zensuke Ota

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to study the three-dimensional ultrastructure of the glomerular basement membrane (GBM), isolated GBM was treated with elastase, sodium dodecyl sulfate, and 2-mercaptoethanol and examined by transmission electron microscopy using ultrathin sectioning method and stereoscopic view. This treatment revealed clearly three-dimensional meshwork structure of GBM mainly composed of type IV collagen. Numerous regular polygonal small pores were present in the meshwork structure. The average short dimension of the small pores was 4.1±1.8nm and the average long dimension was 4.8±2.2 nm. Our present results further support the molecular sieve theory of the basement membrane.

Original languageEnglish
Pages (from-to)182-185
Number of pages4
JournalJournal of Electron Microscopy
Volume39
Issue number3
Publication statusPublished - Jun 1 1990

Keywords

  • Chemical treatment
  • Glomerular basement membrane
  • Three-dimensional structure
  • Type IV collagen
  • Ultrastructure

ASJC Scopus subject areas

  • Instrumentation

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