Three-dimensional evaluation of the spinal local neural network revealed by the high-voltage electron microscopy: A double immunohistochemical study

Takumi Oti, Keita Satoh, Kazuhiro Saito, Kazuyoshi Murata, Mitsuhiro Kawata, Tatsuya Sakamoto, Hirotaka Sakamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Three-dimensional (3-D) analysis of anatomical ultrastructures is important in biological research. However, 3-D image analysis on exact serial sets of ultrathin sections from biological specimens is very difficult to achieve, and limited information can be obtained by 3-D reconstruction from these sections due to the small area that can be reconstructed. On the other hand, the highpenetration power of electrons by an ultra-high accelerating voltage enables thick sections of biological specimens to be examined. High-voltage electron microscopy (HVEM) is particularly useful for 3-D analysis of the central nervous system because considerably thick sections can be observed at the ultrastructure level. Here, we applied HVEM tomography assisted by light microscopy to a study of the 3-D chemical neuroanatomy of the rat lower spinal cord annotated by double-labeling immunohistochemistry. This powerful methodology is useful for studying molecular and/or chemical neuroanatomy at the 3-D ultrastructural level.

Original languageEnglish
Pages (from-to)693-697
Number of pages5
JournalHistochemistry and Cell Biology
Volume138
Issue number4
DOIs
Publication statusPublished - Oct 1 2012

Keywords

  • High-voltage electron microscopy
  • Histological methodology
  • Neuroanatomy
  • Three-dimensional analysis
  • Tomography
  • Ultrastructure

ASJC Scopus subject areas

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

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