Three-dimensional cell and tissue patterning in a strained fibrin gel system

Takuya Matsumoto, Jun Ichi Sasaki, Eben Alsberg, Hiroshi Egusa, Hirofumi Yatani, Taiji Sohmura

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cetl patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.

Original languageEnglish
Article numbere1211
JournalPLoS One
Volume2
Issue number11
DOIs
Publication statusPublished - Nov 21 2007
Externally publishedYes

Fingerprint

fibrin
Fibrin
Gels
gels
Tissue
cells
Reproduction
Endothelial cells
Cell proliferation
Biomimetics
biomimetics
tissue engineering
myoblasts
Tissue engineering
Myoblasts
Muscle
Tissue Engineering
endothelial cells
skeletal muscle
Direction compound

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Matsumoto, T., Sasaki, J. I., Alsberg, E., Egusa, H., Yatani, H., & Sohmura, T. (2007). Three-dimensional cell and tissue patterning in a strained fibrin gel system. PLoS One, 2(11), [e1211]. https://doi.org/10.1371/journal.pone.0001211

Three-dimensional cell and tissue patterning in a strained fibrin gel system. / Matsumoto, Takuya; Sasaki, Jun Ichi; Alsberg, Eben; Egusa, Hiroshi; Yatani, Hirofumi; Sohmura, Taiji.

In: PLoS One, Vol. 2, No. 11, e1211, 21.11.2007.

Research output: Contribution to journalArticle

Matsumoto, T, Sasaki, JI, Alsberg, E, Egusa, H, Yatani, H & Sohmura, T 2007, 'Three-dimensional cell and tissue patterning in a strained fibrin gel system', PLoS One, vol. 2, no. 11, e1211. https://doi.org/10.1371/journal.pone.0001211
Matsumoto, Takuya ; Sasaki, Jun Ichi ; Alsberg, Eben ; Egusa, Hiroshi ; Yatani, Hirofumi ; Sohmura, Taiji. / Three-dimensional cell and tissue patterning in a strained fibrin gel system. In: PLoS One. 2007 ; Vol. 2, No. 11.
@article{0945c8aaf3ed4292b605ec5193190c6f,
title = "Three-dimensional cell and tissue patterning in a strained fibrin gel system",
abstract = "Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cetl patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.",
author = "Takuya Matsumoto and Sasaki, {Jun Ichi} and Eben Alsberg and Hiroshi Egusa and Hirofumi Yatani and Taiji Sohmura",
year = "2007",
month = "11",
day = "21",
doi = "10.1371/journal.pone.0001211",
language = "English",
volume = "2",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Three-dimensional cell and tissue patterning in a strained fibrin gel system

AU - Matsumoto, Takuya

AU - Sasaki, Jun Ichi

AU - Alsberg, Eben

AU - Egusa, Hiroshi

AU - Yatani, Hirofumi

AU - Sohmura, Taiji

PY - 2007/11/21

Y1 - 2007/11/21

N2 - Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cetl patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.

AB - Techniques developed for the in vitro reproduction of three-dimensional (3D) biomimetic tissue will be valuable for investigating changes in cell function in tissues and for fabricating cell/matrix composites for applications in tissue engineering techniques. In this study, we show that the simple application of a continuous strain to a fibrin gel facilitates the development of fibril alignment and bundle-like structures in the fibrin gel in the direction of the applied strain. Myoblasts cultured in this gel also exhibited well-aligned cell patterning in a direction parallel to the direction of the strain. Interestingly, the direction of cell proliferation was identical to that of cell alignment. Finally, the oriented cells formed linear groups that were aligned parallel to the direction of the strain and replicated the native skeletal muscle cetl patterning. In addition, vein endothelial cells formed a linear, aligned vessel-like structure in this system. Thus, the system enables the in vitro reproduction of 3D aligned cell sets replicating biological tissue patterns.

UR - http://www.scopus.com/inward/record.url?scp=43249118905&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43249118905&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0001211

DO - 10.1371/journal.pone.0001211

M3 - Article

C2 - 18030345

AN - SCOPUS:43249118905

VL - 2

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e1211

ER -