Three-color flow cytometric study on lymphocytes derived from thymic diseases

Meinoshin Okumura, Yoshitaka Fujii, Shinichiro Miyoshi, Hiroyuki Shiono, Masayoshi Inoue, Yoshihisa Kadota, Kenjirou Fukuhara, Hikaru Matsuda

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background. Anterior mediastinal masses derive from a variety of diseases. Thymomas have been shown to commonly hold CD4+CD8+ double-positive (DP) lymphocytes, and identification of this subset by two-color flow cytometric study was suggested to help diagnosis of thymoma. Several other thymic diseases, however, possibly hold CD4+CD8+ DP lymphocytes. In this study, we utilized the three-color flow cytometric method for further examination of the phenotypes of lymphocytes in the thymic diseases. Materials and methods. One hundred eight specimens (77 primary and 10 metastatic thymomas, 10 thymic carcinomas, 2 thymic carcinoids, 4 malignant lymphomas, 2 seminomas, an inflammatory pseudotumor, and 2 nonneoplastic thymic hyperplasias) were subjected to the study. The expressions of CD3, CD4, and CD8 on tumor-associated lymphocytes were evaluated by three-color flow cytometric study. Results. The proportion of the CD4+CD8+ DP subset was more than 30% in all 78 lymphocyte-rich thymomas, in 2 malignant lymphomas, and in both thymic hyperplasias. CD3 expression of the CD4+CD8+ DP subset ranged from a negative to a high level in thymomas and thymic hyperplasias, while it was restricted to a particular level in CD4+CD8+ DP-type malignant lymphomas. The proportion of CD3+ cells in the CD4+CD8- single-positive subset was consistently less than 90% in the lymphocyte-rich thymomas, while it was more than 90% in the thymic hyperplasias. Conclusion. Although identification of the CD4+CD8+ DP subset in the tumor-associated lymphocytes does not necessarily indicate thymoma, a further characterization of thymic neoplasms possessing the CD4+CD8+ DP subset was enabled by three-color flow cytometric study, suggesting the utility of this method as an anciliary tool for differential diagnosis of these diseases.

Original languageEnglish
Pages (from-to)130-137
Number of pages8
JournalJournal of Surgical Research
Volume101
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Thymoma
Color
Thymus Hyperplasia
Lymphocytes
CD8-Positive T-Lymphocytes
Lymphoma
Thymus Neoplasms
Plasma Cell Granuloma
Seminoma
Lymphocyte Subsets
Carcinoid Tumor
Neoplasms
Differential Diagnosis
Phenotype

Keywords

  • Malignant lymphoma
  • Thymic carcinoma
  • Thymic hyperplasia
  • Thymoma

ASJC Scopus subject areas

  • Surgery

Cite this

Okumura, M., Fujii, Y., Miyoshi, S., Shiono, H., Inoue, M., Kadota, Y., ... Matsuda, H. (2001). Three-color flow cytometric study on lymphocytes derived from thymic diseases. Journal of Surgical Research, 101(2), 130-137. https://doi.org/10.1006/jsre.2001.6282

Three-color flow cytometric study on lymphocytes derived from thymic diseases. / Okumura, Meinoshin; Fujii, Yoshitaka; Miyoshi, Shinichiro; Shiono, Hiroyuki; Inoue, Masayoshi; Kadota, Yoshihisa; Fukuhara, Kenjirou; Matsuda, Hikaru.

In: Journal of Surgical Research, Vol. 101, No. 2, 2001, p. 130-137.

Research output: Contribution to journalArticle

Okumura, M, Fujii, Y, Miyoshi, S, Shiono, H, Inoue, M, Kadota, Y, Fukuhara, K & Matsuda, H 2001, 'Three-color flow cytometric study on lymphocytes derived from thymic diseases', Journal of Surgical Research, vol. 101, no. 2, pp. 130-137. https://doi.org/10.1006/jsre.2001.6282
Okumura, Meinoshin ; Fujii, Yoshitaka ; Miyoshi, Shinichiro ; Shiono, Hiroyuki ; Inoue, Masayoshi ; Kadota, Yoshihisa ; Fukuhara, Kenjirou ; Matsuda, Hikaru. / Three-color flow cytometric study on lymphocytes derived from thymic diseases. In: Journal of Surgical Research. 2001 ; Vol. 101, No. 2. pp. 130-137.
@article{f8b9c2bcde3047338d0eabb37097989c,
title = "Three-color flow cytometric study on lymphocytes derived from thymic diseases",
abstract = "Background. Anterior mediastinal masses derive from a variety of diseases. Thymomas have been shown to commonly hold CD4+CD8+ double-positive (DP) lymphocytes, and identification of this subset by two-color flow cytometric study was suggested to help diagnosis of thymoma. Several other thymic diseases, however, possibly hold CD4+CD8+ DP lymphocytes. In this study, we utilized the three-color flow cytometric method for further examination of the phenotypes of lymphocytes in the thymic diseases. Materials and methods. One hundred eight specimens (77 primary and 10 metastatic thymomas, 10 thymic carcinomas, 2 thymic carcinoids, 4 malignant lymphomas, 2 seminomas, an inflammatory pseudotumor, and 2 nonneoplastic thymic hyperplasias) were subjected to the study. The expressions of CD3, CD4, and CD8 on tumor-associated lymphocytes were evaluated by three-color flow cytometric study. Results. The proportion of the CD4+CD8+ DP subset was more than 30{\%} in all 78 lymphocyte-rich thymomas, in 2 malignant lymphomas, and in both thymic hyperplasias. CD3 expression of the CD4+CD8+ DP subset ranged from a negative to a high level in thymomas and thymic hyperplasias, while it was restricted to a particular level in CD4+CD8+ DP-type malignant lymphomas. The proportion of CD3+ cells in the CD4+CD8- single-positive subset was consistently less than 90{\%} in the lymphocyte-rich thymomas, while it was more than 90{\%} in the thymic hyperplasias. Conclusion. Although identification of the CD4+CD8+ DP subset in the tumor-associated lymphocytes does not necessarily indicate thymoma, a further characterization of thymic neoplasms possessing the CD4+CD8+ DP subset was enabled by three-color flow cytometric study, suggesting the utility of this method as an anciliary tool for differential diagnosis of these diseases.",
keywords = "Malignant lymphoma, Thymic carcinoma, Thymic hyperplasia, Thymoma",
author = "Meinoshin Okumura and Yoshitaka Fujii and Shinichiro Miyoshi and Hiroyuki Shiono and Masayoshi Inoue and Yoshihisa Kadota and Kenjirou Fukuhara and Hikaru Matsuda",
year = "2001",
doi = "10.1006/jsre.2001.6282",
language = "English",
volume = "101",
pages = "130--137",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Three-color flow cytometric study on lymphocytes derived from thymic diseases

AU - Okumura, Meinoshin

AU - Fujii, Yoshitaka

AU - Miyoshi, Shinichiro

AU - Shiono, Hiroyuki

AU - Inoue, Masayoshi

AU - Kadota, Yoshihisa

AU - Fukuhara, Kenjirou

AU - Matsuda, Hikaru

PY - 2001

Y1 - 2001

N2 - Background. Anterior mediastinal masses derive from a variety of diseases. Thymomas have been shown to commonly hold CD4+CD8+ double-positive (DP) lymphocytes, and identification of this subset by two-color flow cytometric study was suggested to help diagnosis of thymoma. Several other thymic diseases, however, possibly hold CD4+CD8+ DP lymphocytes. In this study, we utilized the three-color flow cytometric method for further examination of the phenotypes of lymphocytes in the thymic diseases. Materials and methods. One hundred eight specimens (77 primary and 10 metastatic thymomas, 10 thymic carcinomas, 2 thymic carcinoids, 4 malignant lymphomas, 2 seminomas, an inflammatory pseudotumor, and 2 nonneoplastic thymic hyperplasias) were subjected to the study. The expressions of CD3, CD4, and CD8 on tumor-associated lymphocytes were evaluated by three-color flow cytometric study. Results. The proportion of the CD4+CD8+ DP subset was more than 30% in all 78 lymphocyte-rich thymomas, in 2 malignant lymphomas, and in both thymic hyperplasias. CD3 expression of the CD4+CD8+ DP subset ranged from a negative to a high level in thymomas and thymic hyperplasias, while it was restricted to a particular level in CD4+CD8+ DP-type malignant lymphomas. The proportion of CD3+ cells in the CD4+CD8- single-positive subset was consistently less than 90% in the lymphocyte-rich thymomas, while it was more than 90% in the thymic hyperplasias. Conclusion. Although identification of the CD4+CD8+ DP subset in the tumor-associated lymphocytes does not necessarily indicate thymoma, a further characterization of thymic neoplasms possessing the CD4+CD8+ DP subset was enabled by three-color flow cytometric study, suggesting the utility of this method as an anciliary tool for differential diagnosis of these diseases.

AB - Background. Anterior mediastinal masses derive from a variety of diseases. Thymomas have been shown to commonly hold CD4+CD8+ double-positive (DP) lymphocytes, and identification of this subset by two-color flow cytometric study was suggested to help diagnosis of thymoma. Several other thymic diseases, however, possibly hold CD4+CD8+ DP lymphocytes. In this study, we utilized the three-color flow cytometric method for further examination of the phenotypes of lymphocytes in the thymic diseases. Materials and methods. One hundred eight specimens (77 primary and 10 metastatic thymomas, 10 thymic carcinomas, 2 thymic carcinoids, 4 malignant lymphomas, 2 seminomas, an inflammatory pseudotumor, and 2 nonneoplastic thymic hyperplasias) were subjected to the study. The expressions of CD3, CD4, and CD8 on tumor-associated lymphocytes were evaluated by three-color flow cytometric study. Results. The proportion of the CD4+CD8+ DP subset was more than 30% in all 78 lymphocyte-rich thymomas, in 2 malignant lymphomas, and in both thymic hyperplasias. CD3 expression of the CD4+CD8+ DP subset ranged from a negative to a high level in thymomas and thymic hyperplasias, while it was restricted to a particular level in CD4+CD8+ DP-type malignant lymphomas. The proportion of CD3+ cells in the CD4+CD8- single-positive subset was consistently less than 90% in the lymphocyte-rich thymomas, while it was more than 90% in the thymic hyperplasias. Conclusion. Although identification of the CD4+CD8+ DP subset in the tumor-associated lymphocytes does not necessarily indicate thymoma, a further characterization of thymic neoplasms possessing the CD4+CD8+ DP subset was enabled by three-color flow cytometric study, suggesting the utility of this method as an anciliary tool for differential diagnosis of these diseases.

KW - Malignant lymphoma

KW - Thymic carcinoma

KW - Thymic hyperplasia

KW - Thymoma

UR - http://www.scopus.com/inward/record.url?scp=0035674763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035674763&partnerID=8YFLogxK

U2 - 10.1006/jsre.2001.6282

DO - 10.1006/jsre.2001.6282

M3 - Article

C2 - 11735267

AN - SCOPUS:0035674763

VL - 101

SP - 130

EP - 137

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -