Thermoradiotherapy combined with p53 gene therapy of human salivary gland adenocarcinoma cell line

Jun-Ichi Asaumi, Yuzuru Higuchi, Jun Murakami, Hidenobu Matsuzaki, Toru Wakasa, Tetsuyoshi Inoue, Hiroshi Shigehara, Hironobu Konouchi, Miki Hisatomi, Shoji Kawasaki, Masahiro Kuroda, Yoshio Hiraki, Kanji Kishi

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Abstract

We report on thermoradiotherapy combined with p53 gene therapy in the human salivary gland adenocarcinoma cell line HSG. HSG cells were successfully infected at a rate of 62.3% with MOIs of 20 of either AxCAip53 or AxCAiLacZ. The AxCAiLacZ did not inhibit the survival of the HSG cells. The survival fractions of AxCAip53-infected HSG cells were lower than those of the AxCAiLacZ-infected HSG cells, but there was no significant difference (p=0.30). AxCAip53 decreased the survival rates of thermotherapy (43°C; p=0.084 and 44°C; p=0.18), radiotherapy (6 Gy; p=0.20) and thermoradiotherapy (6 Gy plus 43°C; p=0.24 and 6 Gy plus 44°C; p=0.96), but there were no significant differences. In comparing the survival rates of thermoradiotherapy with those of thermotherapy and radiotherapy, thermoradiotherapy, regardless of the combination with p53 gene therapy, demonstrated actual survival rates lower than theoretical survival rates based on survival rates of thermotherapy multiplied by survival rates of radiotherapy. This result indicates that thermoradiotherapy is effective in the treatment of HSG cells. Thermoradiotherapy combined with p53 gene therapy was the most effective therapy among the combinations of therapies demonstrating that thermoradiotherapy combined with p53 gene therapy may be a useful tool in the treatment of HSG cells.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalOncology Reports
Volume10
Issue number1
Publication statusPublished - Jan 2003

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Keywords

  • Adenocarcinoma
  • p53
  • Radiotherapy
  • Salivary gland tumor
  • Thermotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Asaumi, J-I., Higuchi, Y., Murakami, J., Matsuzaki, H., Wakasa, T., Inoue, T., Shigehara, H., Konouchi, H., Hisatomi, M., Kawasaki, S., Kuroda, M., Hiraki, Y., & Kishi, K. (2003). Thermoradiotherapy combined with p53 gene therapy of human salivary gland adenocarcinoma cell line. Oncology Reports, 10(1), 71-74.