Thermoluminescence evidence for light-induced oxidation of tyrosine and histidine residues in manganese-depleted photosystem II particles

Suleyman I. Allakhverdiev, Vyacheslav V. Klimov, Sandor Demeter

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In the thermoluminescence (TL) glow curve of photosystem II, particles depleted of manganese, a tyrosine modifier, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) a bolishes the TL band appearing around -55°C (TL-55). Addition of a histidine modifier, diethylpyrocarbonate results in the disappearance of the band peaking around -30°C (TL-30). NBD treatment also abolishes the EPR signal IIfast of oxidized tyrosine donor, Y21 and inhibits the electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. It is concluded that the TL-55 and TL-30 bands can be assigned to oxidized tyrosine (Y2+) and histidine (His+) residues, respectively, which participate in electron transfer from manganese to the reaction center of chlorophyll, P680+.

Original languageEnglish
Pages (from-to)51-54
Number of pages4
JournalFEBS Letters
Issue number1-2
Publication statusPublished - Feb 3 1992



  • Electron transport
  • Histidine
  • Manganese-depletion
  • Photosystem II
  • Thermoluminescence
  • Tyrosine

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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