TY - JOUR
T1 - Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II
AU - Cui-Yan, Tan
AU - Chun-He, Xu
AU - Jun-Ren, Shen
AU - Shinsuke, Sakuma
AU - Yasushi, Yamamoto
AU - Claude, Balny
AU - Kang-Cheng, Ruan
PY - 2003/8/22
Y1 - 2003/8/22
N2 - The unfolding of 23kD (P23k) protein isolated from spinach photosystem II particle was studied by high pressure and fluorescence spectroscopy. The thermal equilibrium study indicated that the protein could be totally unfolded by 180 or 160 MPa at 20°C and 3°C, respectively. The standard free energy and standard volume change of the protein for unfolding at 20°C is 23.45 kJ/mol and -150.3 ml/mol, respectively. Kinetics study indicated that at 20°C the activation volume for unfolding, Δ Vu ‡, was negative (-66.2 ml/mol), meanwhile the activation volume for folding, Δ Vf‡, was positive (84. 1 ml/mol). The rate constants for folding and unfolding (K0f, K 0u) were 1.87 s-1 and 1.3 × 10-4 s -1, respectively, these results provide some clues to explain why the protein is so sensitive to pressure.
AB - The unfolding of 23kD (P23k) protein isolated from spinach photosystem II particle was studied by high pressure and fluorescence spectroscopy. The thermal equilibrium study indicated that the protein could be totally unfolded by 180 or 160 MPa at 20°C and 3°C, respectively. The standard free energy and standard volume change of the protein for unfolding at 20°C is 23.45 kJ/mol and -150.3 ml/mol, respectively. Kinetics study indicated that at 20°C the activation volume for unfolding, Δ Vu ‡, was negative (-66.2 ml/mol), meanwhile the activation volume for folding, Δ Vf‡, was positive (84. 1 ml/mol). The rate constants for folding and unfolding (K0f, K 0u) were 1.87 s-1 and 1.3 × 10-4 s -1, respectively, these results provide some clues to explain why the protein is so sensitive to pressure.
KW - Fluorescence spectroscopy
KW - High pressure technique
KW - Protein unfolding
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M3 - Article
C2 - 12883641
AN - SCOPUS:0042071196
VL - 35
SP - 677
EP - 681
JO - Acta Biochimica et Biophysica Sinica
JF - Acta Biochimica et Biophysica Sinica
SN - 1672-9145
IS - 7
ER -