Thermal stability of immobilized lipase from candida antarctica in glycerols with various water contents at elevated temperatures

Takashi Kobayashi, Takemasa Matsuo, Yukitaka Kimura, Shuji Adachi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The immobilized lipase from Candida antarctica (fraction B, CALB) was incubated in glycerols with various water contents at 80-100 °C to measure the residual activity as a function of time. The glycerol-containing water stabilized the immobilized CALB, especially at 30-60 wt% water contents. The thermal inactivation behaviors of the immobilized CALB were expressed by a model in which the free energy of activation for the inactivation of the immobilized lipase molecules obeyed a Gaussian distribution.

Original languageEnglish
Pages (from-to)1041-1044
Number of pages4
JournalJAOCS, Journal of the American Oil Chemists' Society
Volume85
Issue number11
DOIs
Publication statusPublished - Nov 1 2008
Externally publishedYes

Keywords

  • Candida antarctica fraction B lipase
  • Gaussian distribution
  • Glycerol
  • Immobilization
  • Thermal inactivation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Organic Chemistry

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