Thermal enhancement of the effect of ifosfamide against a spontaneous murine fibrosarcoma, PSa-II

Masahiro Kuroda, M. Urano, R. Reynolds

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of hyperthermia on the cytotoxicity of 3-(2-chloroethyl)-2-[(2-chloroethyl)amino]-tetrahydro-2H-1,3,2-oxazaphosphorine -2-oxide, ifosfamide (IFO) was investigated in vivo. Tumours were early generation isotransplants of a spontaneous C3Hf/Sed mouse fibrosarcoma. FSa-II. The tumour cell suspensions containing ~ 2 X 105 cells were transplanted into the dorsal site of the C3Hf/Sed mouse foot. Hyperthermia was given by immersing the tumour-bearing foot into a constant temperature water bath set at 41.5°C for 0-90 min when tumours reached 34mm3. IFO was administered ip immediately before hyperthermia. Tumour response was studied by the tumour growth (TG) time assay; namely, the TG time or the time for one-half of the treated tumours to reach 700 mm3 from the initial treatment day was determined and the dose-response curve was fitted between the TG time and IFO dose. The anti-tumour effect of IFO was enhanced at this elevated temperature. The thermal enhancement ratio (TER) or the ratio of the slope of dose-response curve at 41.5°C to that of dose-response curve without hyperthermia was relatively small for a short treatment time of 30 min. This TER was smaller for IFO than the TERs for cyclophosphamide (CY) and BCNU which had been studied in our laboratory. However, the TER for IFO increased greatly with a prolongation of treatment time from 30 to 90 min, and exceeded the TER for CY. The TERs were 1.5, 2.6 and 3.6 for heating time of 30, 60, and 90 min, respectively, indicating that a long treatment time such as 90 min at moderately elevated temperatures could result in a substantial enhancement of the antitumour effect of IFO.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalInternational Journal of Hyperthermia
Volume13
Issue number1
Publication statusPublished - Jan 1997

Fingerprint

Ifosfamide
Fibrosarcoma
Hot Temperature
Neoplasms
Fever
Cyclophosphamide
Temperature
Foot
Growth
Isografts
Carmustine
Therapeutics
Baths
Heating
Oxides
Suspensions

Keywords

  • FSa-II cells
  • Ifosfamide
  • Thermal enhancement ratio
  • Thermochemotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Radiological and Ultrasound Technology

Cite this

Thermal enhancement of the effect of ifosfamide against a spontaneous murine fibrosarcoma, PSa-II. / Kuroda, Masahiro; Urano, M.; Reynolds, R.

In: International Journal of Hyperthermia, Vol. 13, No. 1, 01.1997, p. 125-131.

Research output: Contribution to journalArticle

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