Abstract
Time-dependent influence of glial cell line-derived neurotrophic factor (GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with GDNF significantly reduced the infarct volume stained with 2,3,5-triphenyltetrazolium chloride (TTC) when GDNF was topically applied at 0 and 1 h of reperfusion, but became insignificant at 3 h as compared to vehicle group. The protective effect of GDNF was closely related to the significant reduction of the number of TUNEL positive cells as well as immunofluorescently positive cells for active forms of caspases, especially active caspase-3 but not -9. Thus, the present study showed that topical application of GDNF significantly reduced infarct size in a time-dependent manner, while the therapeutic time window was shorter than other chemical compounds such as an NMDA receptor antagonist (MK-801) and a free radical scavenger (PBN). The effect of GDNF was stronger in suppressing active caspase-3 than active caspase-9.
| Original language | English |
|---|---|
| Pages (from-to) | 259-264 |
| Number of pages | 6 |
| Journal | International Congress Series |
| Volume | 1252 |
| Issue number | C |
| DOIs | |
| Publication status | Published - Jun 1 2003 |
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Keywords
- Caspase-3
- Caspase-9
- GDNF
- Ischemia
- TUNEL
ASJC Scopus subject areas
- Medicine(all)
Cite this
Therapeutic time window of glial cell line-derived neurotrophic factor after transient middle cerebral artery occlusion in rat. / Zhang, W. R.; Sato, K.; Iwai, M.; Ota, K.; Abe, Koji.
In: International Congress Series, Vol. 1252, No. C, 01.06.2003, p. 259-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Therapeutic time window of glial cell line-derived neurotrophic factor after transient middle cerebral artery occlusion in rat
AU - Zhang, W. R.
AU - Sato, K.
AU - Iwai, M.
AU - Ota, K.
AU - Abe, Koji
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Time-dependent influence of glial cell line-derived neurotrophic factor (GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with GDNF significantly reduced the infarct volume stained with 2,3,5-triphenyltetrazolium chloride (TTC) when GDNF was topically applied at 0 and 1 h of reperfusion, but became insignificant at 3 h as compared to vehicle group. The protective effect of GDNF was closely related to the significant reduction of the number of TUNEL positive cells as well as immunofluorescently positive cells for active forms of caspases, especially active caspase-3 but not -9. Thus, the present study showed that topical application of GDNF significantly reduced infarct size in a time-dependent manner, while the therapeutic time window was shorter than other chemical compounds such as an NMDA receptor antagonist (MK-801) and a free radical scavenger (PBN). The effect of GDNF was stronger in suppressing active caspase-3 than active caspase-9.
AB - Time-dependent influence of glial cell line-derived neurotrophic factor (GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with GDNF significantly reduced the infarct volume stained with 2,3,5-triphenyltetrazolium chloride (TTC) when GDNF was topically applied at 0 and 1 h of reperfusion, but became insignificant at 3 h as compared to vehicle group. The protective effect of GDNF was closely related to the significant reduction of the number of TUNEL positive cells as well as immunofluorescently positive cells for active forms of caspases, especially active caspase-3 but not -9. Thus, the present study showed that topical application of GDNF significantly reduced infarct size in a time-dependent manner, while the therapeutic time window was shorter than other chemical compounds such as an NMDA receptor antagonist (MK-801) and a free radical scavenger (PBN). The effect of GDNF was stronger in suppressing active caspase-3 than active caspase-9.
KW - Caspase-3
KW - Caspase-9
KW - GDNF
KW - Ischemia
KW - TUNEL
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U2 - 10.1016/S0531-5131(03)00018-9
DO - 10.1016/S0531-5131(03)00018-9
M3 - Article
AN - SCOPUS:85023082757
VL - 1252
SP - 259
EP - 264
JO - International Congress Series
JF - International Congress Series
SN - 0531-5131
IS - C
ER -