Abstract
The time dependent influence of adenovirus-mediated glial cell line-derived neurotrophic factor (GDNF) gene (Ad-GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with Ad-GDNF significantly reduced the infarct volume when immediately administered after the reperfusion, but became insignificant when administered at 1 h after the reperfusion as were the cases treated with vehicle- and adenoviral vector containing the E. coli lacZ gene (Ad-LacZ)-treated groups. The protective effect of GDNF was related to the significant reduction of the number of TUNEL positive cells as well as immunohistochemical positive cells for active caspase-3 but not -9. These results showed that exogenous GDNF gene transfer successfully reduced the infarct size in a time-dependant manner by suppressing active caspase-3 but not active caspase-9. However, the therapeutic time window was shorter than the effect of GDNF protein itself previously reported.
| Original language | English |
|---|---|
| Pages (from-to) | 140-145 |
| Number of pages | 6 |
| Journal | Brain Research |
| Volume | 947 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 23 2002 |
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Keywords
- Ischemia
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat. / Zhang, W. R.; Sato, K.; Iwai, M.; Nagano, I.; Manabe, Y.; Abe, Koji.
In: Brain Research, Vol. 947, No. 1, 23.08.2002, p. 140-145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat
AU - Zhang, W. R.
AU - Sato, K.
AU - Iwai, M.
AU - Nagano, I.
AU - Manabe, Y.
AU - Abe, Koji
PY - 2002/8/23
Y1 - 2002/8/23
N2 - The time dependent influence of adenovirus-mediated glial cell line-derived neurotrophic factor (GDNF) gene (Ad-GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with Ad-GDNF significantly reduced the infarct volume when immediately administered after the reperfusion, but became insignificant when administered at 1 h after the reperfusion as were the cases treated with vehicle- and adenoviral vector containing the E. coli lacZ gene (Ad-LacZ)-treated groups. The protective effect of GDNF was related to the significant reduction of the number of TUNEL positive cells as well as immunohistochemical positive cells for active caspase-3 but not -9. These results showed that exogenous GDNF gene transfer successfully reduced the infarct size in a time-dependant manner by suppressing active caspase-3 but not active caspase-9. However, the therapeutic time window was shorter than the effect of GDNF protein itself previously reported.
AB - The time dependent influence of adenovirus-mediated glial cell line-derived neurotrophic factor (GDNF) gene (Ad-GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with Ad-GDNF significantly reduced the infarct volume when immediately administered after the reperfusion, but became insignificant when administered at 1 h after the reperfusion as were the cases treated with vehicle- and adenoviral vector containing the E. coli lacZ gene (Ad-LacZ)-treated groups. The protective effect of GDNF was related to the significant reduction of the number of TUNEL positive cells as well as immunohistochemical positive cells for active caspase-3 but not -9. These results showed that exogenous GDNF gene transfer successfully reduced the infarct size in a time-dependant manner by suppressing active caspase-3 but not active caspase-9. However, the therapeutic time window was shorter than the effect of GDNF protein itself previously reported.
KW - Ischemia
UR - http://www.scopus.com/inward/record.url?scp=0037162971&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037162971&partnerID=8YFLogxK
U2 - 10.1016/S0006-8993(02)02923-2
DO - 10.1016/S0006-8993(02)02923-2
M3 - Article
C2 - 12144862
AN - SCOPUS:0037162971
VL - 947
SP - 140
EP - 145
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -