Therapeutic time window of Ad-GDNF after transient middle cerebral artery occlusion in rat

W. R. Zhang, K. Sato, M. Iwai, I. Nagano, K. Abe

Research output: Contribution to journalArticlepeer-review

Abstract

Time-dependent influence of adenovirus-mediated glial cell line-derived neurotrophic factor (GDNF) gene (Ad-GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with Ad-GDNF significantly reduced the infarct volume when immediately administered after reperfusion, but became insignificant when administered at 1 h after the reperfusion as were the cases treated with vehicle and adenoviral vector containing the Escherichia coli lacZ gene (Ad-LacZ)-treated groups. The protective effect of GDNF was related to the significant reduction of the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL)-positive cells as well as immunohistochemical positive cells for active caspase-3 but not caspase-9. These results showed that exogenous GDNF gene transfer successfully reduced the infarct size in a time-dependant manner by suppressing active caspase-3 but not active caspase-9. However, the therapeutic time window was shorter than the effect of GDNF protein itself previously reported.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalInternational Congress Series
Volume1252
Issue numberC
DOIs
Publication statusPublished - Jun 1 2003

Keywords

  • Caspase-3
  • Caspase-9
  • GDNF
  • Ischemia
  • TUNEL

ASJC Scopus subject areas

  • Medicine(all)

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