The yellow variegated (var2) locus encodes a homologue of FtsH, an ATP-dependent protease in arabidopsis

Katsuaki Takechi, Sodmergen, Minoru Murata, Fusao Motoyoshi, Wataru Sakamoto

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Variegated leaves are often caused by a nuclear recessive mutation in higher plants. Characterization of the gene responsible for variegation has shown to provide several pathways involved in plastid differentiation. Here we describe an Arabidopsis variegated mutant isolated by T-DNA tagging. The mutant displayed green and yellow sectors in all green tissues except for cotyledons. Cells in the yellow sector of the mutant contained both normal-appearing and mutant chloroplasts. The isolated mutant was shown to be an allele of the previously reported mutant, yellow variegated (var2). Cloning and molecular characterization of the VAR2 locus revealed that it potentially encodes a chloro plastic homologue of FtsH, an ATP-dependent metalloprotease that belongs to a large protein family involved in various cellular functions. ftsH-like genes appear to comprise a small gene family in Arabidopsis genome, since at least six homologues were found in addition to VAR2. Dispensability of VAR2 was therefore explained by the redundancy of genes coding for FstHs. In the yellow regions of the mutant leaves, accumulation of photosynthetic protein components in the thylakoid membrane appeared to be impaired. Based on the role of FtsH in a protein degradation pathway in plastids, we propose a possibility that VAR2 is required for plastid differentiation by avoiding partial photooxidation of developing chloroplasts.

Original languageEnglish
Pages (from-to)1334-1346
Number of pages13
JournalPlant and Cell Physiology
Volume41
Issue number12
Publication statusPublished - 2000

Fingerprint

ATP-Dependent Proteases
Arabidopsis
Plastids
proteinases
mutants
loci
Chloroplasts
Genes
plastids
Thylakoids
Cotyledon
Molecular Cloning
Metalloproteases
Proteolysis
Plastics
genes
chloroplasts
Proteins
Adenosine Triphosphate
Alleles

Keywords

  • Arabidopsis thaliana
  • DNA taggin
  • FtsH
  • Plastid
  • T
  • Variegated mutant

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

The yellow variegated (var2) locus encodes a homologue of FtsH, an ATP-dependent protease in arabidopsis. / Takechi, Katsuaki; Sodmergen; Murata, Minoru; Motoyoshi, Fusao; Sakamoto, Wataru.

In: Plant and Cell Physiology, Vol. 41, No. 12, 2000, p. 1334-1346.

Research output: Contribution to journalArticle

Takechi, Katsuaki ; Sodmergen ; Murata, Minoru ; Motoyoshi, Fusao ; Sakamoto, Wataru. / The yellow variegated (var2) locus encodes a homologue of FtsH, an ATP-dependent protease in arabidopsis. In: Plant and Cell Physiology. 2000 ; Vol. 41, No. 12. pp. 1334-1346.
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