The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2

Tomotaka Tanabe, Tatsuya Funahashi, Noriyuki Okajima, Hiroshi Nakao, Yasuo Takeuchi, Katsushiro Miyamoto, Hiroshi Tsujibo, Shigeo Yamamoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We previously reported that the Vibrio parahaemolyticus pvsABCDE and psuA-pvuABCDE operons are involved in the biosynthesis and transport of its own siderophore, vibrioferrin (VF). Of these, psuA and pvuA encode TonB-dependent outer-membrane proteins (OMPs). Although pvuA was characterized as the ferric vibrioferrin receptor gene, the role of the psuA product remains unknown. In this study, a growth assay of isogenic psuA, pvuA, and psuA-pvuA double-deletion mutants followed by complementation of the double-deletion mutant with psuA or pvuA was used to identify psuA as a gene encoding an OMP involved in the uptake of ferric VF. Thus, psuA and pvuA were renamed pvuA1 and pvuA2, respectively. Moreover, we clarified the TonB specificities of PvuA1 and PvuA2, because V. parahaemolyticus has three sets of the TonB systems. The triple deletion of pvuA1, tonB1, and tonB2, and the double deletion of pvuA2 and tonB2 resulted in the complete loss of growth promotion by VF. This finding indicates that the energy required for PvuA1 and PvuA2 to transport ferric VF across the outer membrane is provided by the TonB2 system and by both the TonB1 and TonB2 systems, respectively.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalFEMS Microbiology Letters
Volume324
Issue number1
DOIs
Publication statusPublished - Nov 2011

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Vibrio parahaemolyticus
Genes
Membrane Proteins
Siderophores
Operon
Growth
vibrioferrin
Membranes

Keywords

  • Ferric vibrioferrin receptor
  • Siderophore
  • TonB-dependent outer-membrane receptor
  • Vibrio parahaemolyticus
  • Vibrioferrin-utilization gene cluster

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology

Cite this

The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2. / Tanabe, Tomotaka; Funahashi, Tatsuya; Okajima, Noriyuki; Nakao, Hiroshi; Takeuchi, Yasuo; Miyamoto, Katsushiro; Tsujibo, Hiroshi; Yamamoto, Shigeo.

In: FEMS Microbiology Letters, Vol. 324, No. 1, 11.2011, p. 73-79.

Research output: Contribution to journalArticle

Tanabe, Tomotaka ; Funahashi, Tatsuya ; Okajima, Noriyuki ; Nakao, Hiroshi ; Takeuchi, Yasuo ; Miyamoto, Katsushiro ; Tsujibo, Hiroshi ; Yamamoto, Shigeo. / The Vibrio parahaemolyticus pvuA1 gene (formerly termed psuA) encodes a second ferric vibrioferrin receptor that requires tonB2. In: FEMS Microbiology Letters. 2011 ; Vol. 324, No. 1. pp. 73-79.
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