The use of VIGS technology to study plant-herbivore interactions

Ivan Galis, Meredith C. Schuman, Klaus Gase, Christian Hettenhausen, Markus Hartl, Son T. Dinh, Jianqiang Wu, Gustavo Bonaventure, Ian T. Baldwin

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Plants employ a large variety of defense strategies to resist herbivores, which require transcriptional reprogramming of cells and profound changes in plant metabolism. Due to the large number of genes involved in defense processes, rapid screening strategies are essential for elucidating the contributions of individual genes in the responses of plants to herbivory. However, databases and seed banks of mutant plants which allow rapid retrieval of mutant genotypes are limited to a few model plant species, namely, Arabidopsis thaliana and Oryza sativa (rice). In other plants, virus-induced gene silencing (VIGS) offers an efficient alternative for screening the functions of individual genes in order to prioritize the allocations of the large time investments required to establish stably transformed RNAi-silenced lines. With VIGS, it is usually possible to achieve strong, specific silencing of target genes in the ecological models Nicotiana attenuata and Solanum nigrum , allowing the rapid assessment of gene silencing effects on phytohormone accumulation, signal transduction and accumulation of defense metabolites. VIGS plants are also useful in bioassays with specialist and generalist herbivores, allowing direct verification of gene function in plant resistance to herbivores.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages109-137
Number of pages29
Volume975
ISBN (Print)9781627032773
DOIs
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume975
ISSN (Print)10643745

Fingerprint

Herbivory
Gene Silencing
Viruses
Technology
Solanum nigrum
Genes
Plant Viruses
Plant Growth Regulators
Gene Order
RNA Interference
Arabidopsis
Biological Assay
Tobacco
Signal Transduction
Genotype
Databases

Keywords

  • Herbivory
  • Nicotiana attenuata
  • Plant hormone level measurements
  • Post-transcriptional gene silencing (PTGS)
  • Secondary metabolite profiling
  • Solanum nigrum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Galis, I., Schuman, M. C., Gase, K., Hettenhausen, C., Hartl, M., Dinh, S. T., ... Baldwin, I. T. (2013). The use of VIGS technology to study plant-herbivore interactions. In Methods in Molecular Biology (Vol. 975, pp. 109-137). (Methods in Molecular Biology; Vol. 975). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-278-0_9

The use of VIGS technology to study plant-herbivore interactions. / Galis, Ivan; Schuman, Meredith C.; Gase, Klaus; Hettenhausen, Christian; Hartl, Markus; Dinh, Son T.; Wu, Jianqiang; Bonaventure, Gustavo; Baldwin, Ian T.

Methods in Molecular Biology. Vol. 975 Humana Press Inc., 2013. p. 109-137 (Methods in Molecular Biology; Vol. 975).

Research output: Chapter in Book/Report/Conference proceedingChapter

Galis, I, Schuman, MC, Gase, K, Hettenhausen, C, Hartl, M, Dinh, ST, Wu, J, Bonaventure, G & Baldwin, IT 2013, The use of VIGS technology to study plant-herbivore interactions. in Methods in Molecular Biology. vol. 975, Methods in Molecular Biology, vol. 975, Humana Press Inc., pp. 109-137. https://doi.org/10.1007/978-1-62703-278-0_9
Galis I, Schuman MC, Gase K, Hettenhausen C, Hartl M, Dinh ST et al. The use of VIGS technology to study plant-herbivore interactions. In Methods in Molecular Biology. Vol. 975. Humana Press Inc. 2013. p. 109-137. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-278-0_9
Galis, Ivan ; Schuman, Meredith C. ; Gase, Klaus ; Hettenhausen, Christian ; Hartl, Markus ; Dinh, Son T. ; Wu, Jianqiang ; Bonaventure, Gustavo ; Baldwin, Ian T. / The use of VIGS technology to study plant-herbivore interactions. Methods in Molecular Biology. Vol. 975 Humana Press Inc., 2013. pp. 109-137 (Methods in Molecular Biology).
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