The use of the S haplotype-specific F-box protein gene, SFB, as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot (Prunus mume)

Hisayo Yamane, Koichiro Ushijima, Hidenori Sassa, Ryutaro Tao

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Japanese apricot (Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatible Prunus species. This report identifies the S haplotype-specific F-box protein gene (SFB), a candidate gene for pollen-S, of Japanese apricot, which leads to the development of a molecular typing system for S-haplotype in this fruit species. Both 5′- and 3′-RACE (rapid amplification of cDNA ends) were performed with SFB gene-specific oligonucleotide primers to clone Pm-SFB 1 and Pm-SFB7 of 'Nanko (S1S7)'. As in the case of SFB of other Prunus species, Pm-SFB1 and Pm-SFB 7 showed a high level of S-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with the Pm-SFB probes under low stringency conditions yielded RFLP bands specific to the S1- to S 8-haplotypes as well as a self-compatible Sf-haplotype. A practical usage of SFB as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot is discussed.

Original languageEnglish
Pages (from-to)1357-1361
Number of pages5
JournalTheoretical and Applied Genetics
Volume107
Issue number8
DOIs
Publication statusPublished - Nov 1 2003
Externally publishedYes

Keywords

  • F-box motif
  • Gametophytic self-incompatibility
  • Pollen determinant
  • Prunus mume
  • S-RNase
  • SFB

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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