Abstract
Japanese apricot (Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatible Prunus species. This report identifies the S haplotype-specific F-box protein gene (SFB), a candidate gene for pollen-S, of Japanese apricot, which leads to the development of a molecular typing system for S-haplotype in this fruit species. Both 5′- and 3′-RACE (rapid amplification of cDNA ends) were performed with SFB gene-specific oligonucleotide primers to clone Pm-SFB 1 and Pm-SFB7 of 'Nanko (S1S7)'. As in the case of SFB of other Prunus species, Pm-SFB1 and Pm-SFB 7 showed a high level of S-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with the Pm-SFB probes under low stringency conditions yielded RFLP bands specific to the S1- to S 8-haplotypes as well as a self-compatible Sf-haplotype. A practical usage of SFB as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot is discussed.
Original language | English |
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Pages (from-to) | 1357-1361 |
Number of pages | 5 |
Journal | Theoretical and Applied Genetics |
Volume | 107 |
Issue number | 8 |
DOIs | |
Publication status | Published - Nov 1 2003 |
Externally published | Yes |
Keywords
- F-box motif
- Gametophytic self-incompatibility
- Pollen determinant
- Prunus mume
- S-RNase
- SFB
ASJC Scopus subject areas
- Biotechnology
- Agronomy and Crop Science
- Genetics