The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions

Marcel P. Beier, Takayuki Fujita, Kazuhiro Sasaki, Keiichi Kanno, Miwa Ohashi, Wataru Tamura, Noriyuki Konishi, Masahide Saito, Fumi Imagawa, Keiki Ishiyama, Akio Miyao, Tomoyuki Yamaya, Soichi Kojima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nitrogen is one of the most important elements for plant growth, and urea is one of the most frequently used nitrogen fertilizers worldwide. Besides the exogenously-supplied urea to the soil, urea is endogenously synthesized during secondary nitrogen metabolism. Here, we investigated the contribution of a urea transporter, DUR3, to rice production using a reverse genetic approach combined with localization studies. Tos17 insertion lines for DUR3 showed a 50% yield reduction in hydroponic culture, and a 26.2% yield reduction in a paddy field, because of decreased grain filling. Because shoot biomass production and shoot total N was not reduced, insertion lines were disordered not only in nitrogen acquisition but also in nitrogen allocation. During seed development, DUR3 insertion lines accumulated nitrogen in leaves and could not sufficiently develop their panicles, although shoot and root dry weights were not significantly different from the wild-type. The urea concentration in old leaf harvested from DUR3 insertion lines was lower than that in wild-type. DUR3 promoter-dependent β-glucuronidase (GUS) activity was localized in vascular tissue and the midribs of old leaves. These results indicate that DUR3 contributes to nitrogen translocation and rice yield under nitrogen-deficient and field conditions.

Original languageEnglish
JournalPhysiologia Plantarum
DOIs
Publication statusAccepted/In press - Jan 1 2019
Externally publishedYes

Fingerprint

transporters
Nitrogen
urea
rice
nitrogen
Urea
shoots
leaves
nitrogen metabolism
vascular tissues
Hydroponics
Secondary Metabolism
seed development
filling period
hydroponics
Reverse Genetics
paddies
urea transporter
Oryza
biomass production

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Beier, M. P., Fujita, T., Sasaki, K., Kanno, K., Ohashi, M., Tamura, W., ... Kojima, S. (Accepted/In press). The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions. Physiologia Plantarum. https://doi.org/10.1111/ppl.12872

The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions. / Beier, Marcel P.; Fujita, Takayuki; Sasaki, Kazuhiro; Kanno, Keiichi; Ohashi, Miwa; Tamura, Wataru; Konishi, Noriyuki; Saito, Masahide; Imagawa, Fumi; Ishiyama, Keiki; Miyao, Akio; Yamaya, Tomoyuki; Kojima, Soichi.

In: Physiologia Plantarum, 01.01.2019.

Research output: Contribution to journalArticle

Beier, MP, Fujita, T, Sasaki, K, Kanno, K, Ohashi, M, Tamura, W, Konishi, N, Saito, M, Imagawa, F, Ishiyama, K, Miyao, A, Yamaya, T & Kojima, S 2019, 'The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions', Physiologia Plantarum. https://doi.org/10.1111/ppl.12872
Beier, Marcel P. ; Fujita, Takayuki ; Sasaki, Kazuhiro ; Kanno, Keiichi ; Ohashi, Miwa ; Tamura, Wataru ; Konishi, Noriyuki ; Saito, Masahide ; Imagawa, Fumi ; Ishiyama, Keiki ; Miyao, Akio ; Yamaya, Tomoyuki ; Kojima, Soichi. / The urea transporter DUR3 contributes to rice production under nitrogen-deficient and field conditions. In: Physiologia Plantarum. 2019.
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