The UL14 protein of herpes simplex virus type 2 translocates the minor capsid protein VP26 and the DNA cleavage and packaging UL33 protein into the nucleus of coexpressing cells

Y. Yamauchi, K. Wada, F. Goshima, H. Takakuwa, T. Daikoku, Masao Yamada, Y. Nishiyama

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The herpes simples virus type 2 (HSV-2) gene UL 14 encodes a 32 kDa protein which is a minor component of the virion tegument and is expressed late in infection. The UL14 protein shows varied localization patterns in HSV-2-infected and singly expressing cells, suggesting the possibility that it is multifunctional. We have investigated the influence of the UL14 protein on the intracellular localization of capsid protiens and DNA cleavage and packaging proteins in coexpressing cells. VP26 is the minor capsid protein; it binds to hexons of the outer capsid shell and is predominantly cytoplasmic upon sole expression. We have found that VP26 coexpressed with the UL14 protien showed mutual and predominant relocation into the nucleus. At least seven viral genes encode protiens (UL6, UL15, UL17, UL25, UL28 and UL33) that are required for DNA cleavage and packaging. We have found that the UL33 protein, which was also cytoplasmic by sole expression, was relocated to the nucleus upon expression with the UL14 protein, which again seemed to be a result of mutual influence. Coexpression experiments also suggested the possibility of a mutual influence between the UL14 and UL17 proteins, and the UL17 protien and VP26. Our results suggest that the UL14 protein can influence the intracellular localization patterns of a number of proteins belonging to the capsid or the DNA encapsidation machinery.

Original languageEnglish
Pages (from-to)321-330
Number of pages10
JournalJournal of General Virology
Volume82
Issue number2
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

DNA Packaging
DNA Cleavage
Human Herpesvirus 2
Capsid Proteins
Cell Nucleus
Proteins
Capsid
Viruses
Human herpesvirus 1 UL14 protein
Viral Genes
Virion

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

The UL14 protein of herpes simplex virus type 2 translocates the minor capsid protein VP26 and the DNA cleavage and packaging UL33 protein into the nucleus of coexpressing cells. / Yamauchi, Y.; Wada, K.; Goshima, F.; Takakuwa, H.; Daikoku, T.; Yamada, Masao; Nishiyama, Y.

In: Journal of General Virology, Vol. 82, No. 2, 2001, p. 321-330.

Research output: Contribution to journalArticle

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T1 - The UL14 protein of herpes simplex virus type 2 translocates the minor capsid protein VP26 and the DNA cleavage and packaging UL33 protein into the nucleus of coexpressing cells

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AU - Wada, K.

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AU - Takakuwa, H.

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AU - Yamada, Masao

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