The tumor suppressor Lats2 is pivotal in Aurora A and Aurora B signaling during mitosis

Norikazu Yabuta, Satomi Mukai, Nobuhiro Okada, Yael Aylon, Hiroshi Nojima

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Accurate coordination between chromosome segregation and cytokinesis by various mitotic kinases, such as Aurora, prevent tetraploidization and subsequent tumorigensis. The tumor suppressors Lats1 and Lats2 are serine/threonine kinases that localize to the centrosome and regulate cell cycle progression and apoptosis. In the present study, Aurora A was demonstrated to phosphorylate Lats2 on serine 380 (S380) during mitosis. Immunocytochemical observations revealed that the subcellular localization of Lats2 was distinct during the cell cycle and depended on which site was phosphorylated. Interestingly, the S380-phosphorylated Lats2 protein (pS380) colocalized at the central spindle with Aurora B. Physical interactions were observed between Aurora A, Lats2, Lats1 and Aurora B. The Lats1 kinase was shown to phosphorylate Aurora B. Cells expressing a nonphosphorylated mutant (S380A) of Lats2 caused chromosome missegregation and cytokinesis failure, similar to the cells with aberrantly expressed Aurora B. Together, the results suggest that the Aurora A-Lats1/2-Aurora B axis might be a novel pathway that regulates accurate mitotic progression by ensuring the proper mitotic localization of Lats2.

Original languageEnglish
Pages (from-to)2724-2736
Number of pages13
JournalCell Cycle
Volume10
Issue number16
DOIs
Publication statusPublished - Aug 15 2011

Keywords

  • Aurora-A
  • Aurora-B
  • Lats1
  • Lats2
  • Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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