The tumor-educated-macrophage increase of malignancy of human pancreatic cancer is prevented by zoledronic acid

Yukihiko Hiroshima, Ali Maawy, Mohamed K. Hassanein, Rhiana Menen, Masashi Momiyama, Takashi Murakami, Shinji Miwa, Mako Yamamoto, Fuminari Uehara, Shuya Yano, Ryutaro Mori, Ryusei Matsuyama, Takashi Chishima, Kuniya Tanaka, Yasushi Ichikawa, Michael Bouvet, Itaru Endo, Robert M. Hoffman

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18 Citations (Scopus)

Abstract

We previously defined macrophages harvested from the peritoneal cavity of nude mice with subcutaneous human pancreatic tumors as "tumor-educated- macrophages" (Edu) and macrophages harvested from mice without tumors as "naïve-macrophages" (Naïve), and demonstrated that Edu-macrophages promoted tumor growth and metastasis. In this study, Edu- and Naïve-macrophages were compared for their ability to enhance pancreatic cancer malignancy at the cellular level in vitro and in vivo. The inhibitory efficacy of Zoledronic acid (ZA) on Edu-macrophage-enhanced metastasis was also determined. XPA1 human pancreatic cancer cells in Gelfoam co-cultured with Edu-macrophages proliferated to a greater extent compared to XPA1 cells cultured with Naïve-macrophages (P = 0.014). XPA1 cells exposed to conditioned medium harvested from Edu culture significantly increased proliferation (P = 0.016) and had more migration stimulation capability (P<0.001) compared to cultured cancer cells treated with the conditioned medium from Naïve. The mitotic index of the XPA1 cells, expressing GFP in the nucleus and RFP in the cytoplasm, significantly increased in vivo in the presence of Educompared to Naïve-macrophages (P = 0.001). Zoledronic acid (ZA) killed both Edu and Naïve in vitro. Edu promoted tumor growth and metastasis in an orthotopic mouse model of the XPA1 human pancreatic cancer cell line. ZA reduced primary tumor growth (P = 0.006) and prevented metastasis (P = 0.025) promoted by Edu-macrophages. These results indicate that ZA inhibits enhanced primary tumor growth and metastasis of human pancreatic cancer induced by Edu-macrophages.

Original languageEnglish
Article numbere103382
JournalPloS one
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 12 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Hiroshima, Y., Maawy, A., Hassanein, M. K., Menen, R., Momiyama, M., Murakami, T., Miwa, S., Yamamoto, M., Uehara, F., Yano, S., Mori, R., Matsuyama, R., Chishima, T., Tanaka, K., Ichikawa, Y., Bouvet, M., Endo, I., & Hoffman, R. M. (2014). The tumor-educated-macrophage increase of malignancy of human pancreatic cancer is prevented by zoledronic acid. PloS one, 9(8), [e103382]. https://doi.org/10.1371/journal.pone.0103382