The tryptophan cluster: A hypothetical structure of the DNA-binding domain of the myb protooncogene product

Chie Kanei-Ishii, Akinori Sarai, Tetsuya Sawazaki, Hideki Nakagoshi, Dong Ning He, Kazuhiro Ogata, Yoshifumi Nishimura, Shunsuke Ishii

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

In the DNA-binding domain of the c-myb protooncogene product (c-Myb) which consists of three repeats of 51-52 amino acids, there are 3 perfectly conserved tryptophans in each repeat. Site-directed mutagenesis of these tryptophans showed that any single or multiple mutations of tryptophan to hydrophilic residues or alanine abolished or greatly reduced the sequence-specific DNA-binding activity, but mutations to hydrophobic amino acids retained considerable activity. Raman spectroscopic study showed that these tryptophans were buried in the protein core. These 3 tryptophans are proposed to form a cluster in the hydrophobic core in each repeat. This hypothetical structure is referred to as the "tryptophan cluster," and it may represent a characteristic property of a group of DNA-binding proteins including the myb- and ets-related proteins.

Original languageEnglish
Pages (from-to)19990-19995
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number32
Publication statusPublished - Nov 15 1990
Externally publishedYes

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Tryptophan
DNA
Amino Acids
Mutagenesis
Mutation
DNA-Binding Proteins
Site-Directed Mutagenesis
Alanine
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kanei-Ishii, C., Sarai, A., Sawazaki, T., Nakagoshi, H., He, D. N., Ogata, K., ... Ishii, S. (1990). The tryptophan cluster: A hypothetical structure of the DNA-binding domain of the myb protooncogene product. Journal of Biological Chemistry, 265(32), 19990-19995.

The tryptophan cluster : A hypothetical structure of the DNA-binding domain of the myb protooncogene product. / Kanei-Ishii, Chie; Sarai, Akinori; Sawazaki, Tetsuya; Nakagoshi, Hideki; He, Dong Ning; Ogata, Kazuhiro; Nishimura, Yoshifumi; Ishii, Shunsuke.

In: Journal of Biological Chemistry, Vol. 265, No. 32, 15.11.1990, p. 19990-19995.

Research output: Contribution to journalArticle

Kanei-Ishii, C, Sarai, A, Sawazaki, T, Nakagoshi, H, He, DN, Ogata, K, Nishimura, Y & Ishii, S 1990, 'The tryptophan cluster: A hypothetical structure of the DNA-binding domain of the myb protooncogene product', Journal of Biological Chemistry, vol. 265, no. 32, pp. 19990-19995.
Kanei-Ishii, Chie ; Sarai, Akinori ; Sawazaki, Tetsuya ; Nakagoshi, Hideki ; He, Dong Ning ; Ogata, Kazuhiro ; Nishimura, Yoshifumi ; Ishii, Shunsuke. / The tryptophan cluster : A hypothetical structure of the DNA-binding domain of the myb protooncogene product. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 32. pp. 19990-19995.
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