The tryptophan cluster: A hypothetical structure of the DNA-binding domain of the myb protooncogene product

C. Kanei-Ishii, A. Sarai, T. Sawazaki, H. Nakagoshi, D. N. He, K. Ogata, Y. Nishimura, S. Ishii

Research output: Contribution to journalArticlepeer-review

113 Citations (Scopus)

Abstract

In the DNA-binding domain of the c-myb protooncogene product (c-Myb) which consists of three repeats of 51-52 amino acids, there are 3 perfectly conserved tryptophans in each repeat. Site-directed mutagenesis of these tryptophans showed that any single or multiple mutations of tryptophan to hydrophilic residues or alanine abolished or greatly reduced the sequence-specific DNA-binding activity, but mutations to hydrophobic amino acids retained considerable activity. Raman spectroscopic study showed that these tryptophans were buried in the protein core. These 3 tryptophans are proposed to form a cluster in the hydrophobic core in each repeat. This hypothetical structure is referred to as the 'tryptophan cluster', and it may represent a characteristic property of a group of DNA-binding proteins including the myb- and ets-related proteins.

Original languageEnglish
Pages (from-to)19990-19995
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number32
Publication statusPublished - 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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