Abstract
Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
| Original language | English |
|---|---|
| Pages (from-to) | 347-351 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 355 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Apr 6 2007 |
| Externally published | Yes |
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Keywords
- MALDI-TOF
- Osterix
- RNA helicase A
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The transcriptional factor Osterix directly interacts with RNA helicase A. / Amorim, Bruna Rabelo; Okamura, Hirohiko; Yoshida, Kaya; Qiu, Lihong; Morimoto, Hiroyuki; Haneji, Tatsuji.
In: Biochemical and Biophysical Research Communications, Vol. 355, No. 2, 06.04.2007, p. 347-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The transcriptional factor Osterix directly interacts with RNA helicase A
AU - Amorim, Bruna Rabelo
AU - Okamura, Hirohiko
AU - Yoshida, Kaya
AU - Qiu, Lihong
AU - Morimoto, Hiroyuki
AU - Haneji, Tatsuji
PY - 2007/4/6
Y1 - 2007/4/6
N2 - Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
AB - Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
KW - MALDI-TOF
KW - Osterix
KW - RNA helicase A
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UR - http://www.scopus.com/inward/citedby.url?scp=33847125732&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.01.150
DO - 10.1016/j.bbrc.2007.01.150
M3 - Article
VL - 355
SP - 347
EP - 351
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -