TY - JOUR
T1 - The transcriptional factor Osterix directly interacts with RNA helicase A
AU - Amorim, Bruna Rabelo
AU - Okamura, Hirohiko
AU - Yoshida, Kaya
AU - Qiu, Lihong
AU - Morimoto, Hiroyuki
AU - Haneji, Tatsuji
N1 - Funding Information:
We thank Dr. T. Iwata for help on the analysis of MALDI-TOF mass spectrometry and valuable discussion. We also thank Mrs. E. Sasaki for her skilful technical assistance. This study was supported in part by grants from the Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2007/4/6
Y1 - 2007/4/6
N2 - Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
AB - Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.
KW - MALDI-TOF
KW - Osterix
KW - RNA helicase A
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U2 - 10.1016/j.bbrc.2007.01.150
DO - 10.1016/j.bbrc.2007.01.150
M3 - Article
C2 - 17303075
AN - SCOPUS:33847125732
VL - 355
SP - 347
EP - 351
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -