The transcriptional factor Osterix directly interacts with RNA helicase A

Bruna Rabelo Amorim, Hirohiko Okamura, Kaya Yoshida, Lihong Qiu, Hiroyuki Morimoto, Tatsuji Haneji

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Osterix is an osteoblast-specific transcriptional factor, required for bone formation and osteoblast differentiation. Here, we identified new Osterix interacting factors by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Among the candidates, RNA helicase A was identified to interact with Osterix. To determine the interaction of Osterix with RNA helicase A, immunoprecipitation assay was performed. Western analysis confirmed the association between Osterix and RNA helicase A. Immunocytochemical analysis also showed that Osterix and RNA helicase A were co-localized in HEK 293 cells. Our data suggest that RNA helicase A might be a component of Osterix regulation.

Original languageEnglish
Pages (from-to)347-351
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume355
Issue number2
DOIs
Publication statusPublished - Apr 6 2007
Externally publishedYes

Fingerprint

RNA Helicases
Osteoblasts
HEK293 Cells
Immunoprecipitation
Osteogenesis
Ionization
Mass spectrometry
Assays
Desorption
Mass Spectrometry
Bone
Lasers
Association reactions

Keywords

  • MALDI-TOF
  • Osterix
  • RNA helicase A

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The transcriptional factor Osterix directly interacts with RNA helicase A. / Amorim, Bruna Rabelo; Okamura, Hirohiko; Yoshida, Kaya; Qiu, Lihong; Morimoto, Hiroyuki; Haneji, Tatsuji.

In: Biochemical and Biophysical Research Communications, Vol. 355, No. 2, 06.04.2007, p. 347-351.

Research output: Contribution to journalArticle

Amorim, Bruna Rabelo ; Okamura, Hirohiko ; Yoshida, Kaya ; Qiu, Lihong ; Morimoto, Hiroyuki ; Haneji, Tatsuji. / The transcriptional factor Osterix directly interacts with RNA helicase A. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 355, No. 2. pp. 347-351.
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