The thylakoid carbonic anhydrase associated with photosystem II is the component of inorganic carbon accumulating system in cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare

Marina V. Dudoladova, Elena V. Kupriyanova, Alexandra G. Markelova, Maria P. Sinetova, Suleyman I. Allakhverdiev, Natalia A. Pronina

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Abstract

The organization of carbonic anhydrase (CA) system in halo- and alkaliphilic cyanobacterium Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of putative extracellular α-CA of 60 kDa in the glycocalyx, forming a tight sheath around the cell, and of two intracellular β-CA is reported. We show for the first time that the β-CA of 60 kDa is expressed constitutively and associated with polypeptides of photosystem II (β-CA-PS II). Another soluble β-CA of 25 kDa was induced in low-bicarbonate medium. Induction of synthesis of the latter β-CA was accompanied by an increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of a CO2-concentrating mechanism.

Original languageEnglish
Pages (from-to)616-623
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1767
Issue number6
DOIs
Publication statusPublished - Jun 1 2007

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Keywords

  • Alkaliphilic cyanobacteria
  • Carbonic anhydrase immunolocalization
  • Intracellular pool of C
  • Photosystem II
  • Rhabdoderma lineare
  • Soda lakes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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