The thylakoid carbonic anhydrase associated with photosystem II is the component of inorganic carbon accumulating system in cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare

Marina V. Dudoladova, Elena V. Kupriyanova, Alexandra G. Markelova, Maria P. Sinetova, Suleyman I. Allakhverdiev, Natalia A. Pronina

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    The organization of carbonic anhydrase (CA) system in halo- and alkaliphilic cyanobacterium Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of putative extracellular α-CA of 60 kDa in the glycocalyx, forming a tight sheath around the cell, and of two intracellular β-CA is reported. We show for the first time that the β-CA of 60 kDa is expressed constitutively and associated with polypeptides of photosystem II (β-CA-PS II). Another soluble β-CA of 25 kDa was induced in low-bicarbonate medium. Induction of synthesis of the latter β-CA was accompanied by an increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of a CO2-concentrating mechanism.

    Original languageEnglish
    Pages (from-to)616-623
    Number of pages8
    JournalBiochimica et Biophysica Acta - Bioenergetics
    Volume1767
    Issue number6
    DOIs
    Publication statusPublished - Jun 2007

    Keywords

    • Alkaliphilic cyanobacteria
    • Carbonic anhydrase immunolocalization
    • Intracellular pool of C
    • Photosystem II
    • Rhabdoderma lineare
    • Soda lakes

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Cell Biology

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