The tail nick augments Aeromonas sobria serine protease (ASP) activity in plasma through retarding inhibition by α2-macroglobulin

Yoji Murakami; Yoshihiro Wada; Hidetomo Kobayashi; Makoto Hasegawa; Keinosuke Okamoto; Masatoshi Eto; Takahisa Imamura

doi:10.1016/j.febslet.2012.08.004

The tail nick augments Aeromonas sobria serine protease (ASP) activity in plasma through retarding inhibition by α₂-macroglobulin

Yoji Murakami, Yoshihiro Wada, Hidetomo Kobayashi, Makoto Hasegawa, Keinosuke Okamoto, Masatoshi Eto, Takahisa Imamura

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

ASP is a serine protease secreted by Aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. The pathogen also secretes nASP that has a nick in the carboxy-terminal region. Compared with single-chain ASP (sASP), nASP had near-equivalent activity for small peptide substrates but was less proteolytic. Surprisingly, nASP cleaved proteins more in plasma and was inhibited by human α₂-macroglobulin more slowly than sASP. Retarded inhibition by α₂-macroglobulin allows nASP to keep proteolytic activity for longer in the host and exacerbate disorders at Aeromonas sobria infection sites. nASP may be an evolutional form to augment ASP virulence.

Original language	English
Pages (from-to)	3613-3617
Number of pages	5
Journal	FEBS Letters
Volume	586
Issue number	20
DOIs	https://doi.org/10.1016/j.febslet.2012.08.004
Publication status	Published - Oct 19 2012

Keywords

Aeromonas
Bacteria
Inhibitor
Protease
Virulence
α-Macroglobulin

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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The tail nick augments Aeromonas sobria serine protease (ASP) activity in plasma through retarding inhibition by α₂-macroglobulin. / Murakami, Yoji; Wada, Yoshihiro; Kobayashi, Hidetomo; Hasegawa, Makoto; Okamoto, Keinosuke; Eto, Masatoshi; Imamura, Takahisa.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3613-3617.

Research output: Contribution to journal › Article › peer-review

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title = "The tail nick augments Aeromonas sobria serine protease (ASP) activity in plasma through retarding inhibition by α2-macroglobulin",

abstract = "ASP is a serine protease secreted by Aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. The pathogen also secretes nASP that has a nick in the carboxy-terminal region. Compared with single-chain ASP (sASP), nASP had near-equivalent activity for small peptide substrates but was less proteolytic. Surprisingly, nASP cleaved proteins more in plasma and was inhibited by human α2-macroglobulin more slowly than sASP. Retarded inhibition by α2-macroglobulin allows nASP to keep proteolytic activity for longer in the host and exacerbate disorders at Aeromonas sobria infection sites. nASP may be an evolutional form to augment ASP virulence.",

keywords = "Aeromonas, Bacteria, Inhibitor, Protease, Virulence, α-Macroglobulin",

author = "Yoji Murakami and Yoshihiro Wada and Hidetomo Kobayashi and Makoto Hasegawa and Keinosuke Okamoto and Masatoshi Eto and Takahisa Imamura",

note = "Funding Information: This work was supported by the Japanese Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research (C) (22590363) to T.I.",

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AU - Hasegawa, Makoto

AU - Okamoto, Keinosuke

AU - Eto, Masatoshi

AU - Imamura, Takahisa

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KW - Bacteria

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KW - Virulence

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