The structure of native G-actin

Hui Wang, Robert C. Robinson, Leslie D. Burtnick

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Heat shock proteins act as cytoplasmic chaperones to ensure correct protein folding and prevent protein aggregation. The presence of stoichiometric amounts of one such heat shock protein, Hsp27, in supersaturated solutions of unmodified G-actin leads to crystallization, in preference to polymerization, of the actin. Hsp27 is not evident in the resulting crystal structure. Thus, for the first time, we present the structure of G-actin in a form that is devoid of polymerization-deterring chemical modifications or binding partners, either of which may alter its conformation. The structure contains a calcium ion and ATP within a closed nucleotide-binding cleft, and the D-loop is disordered. This native G-actin structure invites comparison with the current F-actin model in order to understand the structural implications for actin polymerization. In particular, this analysis suggests a mechanism by which the bound cation coordinates conformational change and ATP-hydrolysis.

Original languageEnglish
Pages (from-to)456-465
Number of pages10
JournalCytoskeleton
Volume67
Issue number7
DOIs
Publication statusPublished - Jul 1 2010
Externally publishedYes

Fingerprint

Actins
Polymerization
Heat-Shock Proteins
Adenosine Triphosphate
Protein Folding
Crystallization
Cations
Hydrolysis
Nucleotides
Ions
Calcium
Proteins

Keywords

  • Conformational change
  • F-actin
  • G-actin
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology

Cite this

The structure of native G-actin. / Wang, Hui; Robinson, Robert C.; Burtnick, Leslie D.

In: Cytoskeleton, Vol. 67, No. 7, 01.07.2010, p. 456-465.

Research output: Contribution to journalArticle

Wang, H, Robinson, RC & Burtnick, LD 2010, 'The structure of native G-actin', Cytoskeleton, vol. 67, no. 7, pp. 456-465. https://doi.org/10.1002/cm.20458
Wang, Hui ; Robinson, Robert C. ; Burtnick, Leslie D. / The structure of native G-actin. In: Cytoskeleton. 2010 ; Vol. 67, No. 7. pp. 456-465.
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