The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612

Tadashi Hanafusa, Haruyasu Kinashi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In NF strain 2612 of Streptomyces coelicolor, a giant linear plasmid SCP1 is integrated into the chromosome at the 9 o'clock position. To characterize the integrated structure of SCP1, cloning and sequence analysis of the two junctions between the SCP1 DNA and the chromosomal DNA was carried out. The left junction was revealed to retain an almost intact left terminus of SCP1. On the other hand, the right junction was composed of IS 466, deleting completely the right terminal inverted repeat of SCP1. This junction might have been formed by recombination of two IS 466 elements, one present at the end of the right terminal inverted repeat of SCP1 and one on the chromosome. Based on these results, we have proposed a model for the integration of SCP1 into the chromosome. The unique conjugal transfer of NF strains and the origin of the chromosomal antibiotic biosynthetic genes in Streptomyces species are also discussed in relation to this model.

Original languageEnglish
Pages (from-to)363-368
Number of pages6
JournalMGG Molecular & General Genetics
Volume231
Issue number3
DOIs
Publication statusPublished - Feb 1992
Externally publishedYes

Fingerprint

Streptomyces coelicolor
Terminal Repeat Sequences
Plasmids
Chromosomes
Chromosomes, Human, Pair 9
DNA Transposable Elements
DNA
Streptomyces
Genetic Recombination
Sequence Analysis
Organism Cloning
Anti-Bacterial Agents
Genes

Keywords

  • Giant linear plasmid
  • Integration
  • Invertron
  • NF
  • SCP1

ASJC Scopus subject areas

  • Genetics

Cite this

The structure of an integrated copy of the giant linear plasmid SCP1 in the chromosome of Streptomyces coelicolor 2612. / Hanafusa, Tadashi; Kinashi, Haruyasu.

In: MGG Molecular & General Genetics, Vol. 231, No. 3, 02.1992, p. 363-368.

Research output: Contribution to journalArticle

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