The structure of a 15-stranded actin-like filament from Clostridium botulinum

Fujiet Koh, Akihiro Narita, Lin Jie Lee, Kotaro Tanaka, Yong Zi Tan, Venkata P. Dandey, David Popp, Robert C. Robinson

Research output: Contribution to journalArticle

Abstract

Microfilaments (actin) and microtubules represent the extremes in eukaryotic cytoskeleton cross-sectional dimensions, raising the question of whether filament architectures are limited by protein fold. Here, we report the cryoelectron microscopy structure of a complex filament formed from 15 protofilaments of an actin-like protein. This actin-like ParM is encoded on the large pCBH Clostridium botulinum plasmid. In cross-section, the ~26 nm diameter filament comprises a central helical protofilament surrounded by intermediate and outer layers of six and eight twisted protofilaments, respectively. Alternating polarity of the layers allows for similar lateral contacts between each layer. This filament design is stiffer than the actin filament, and has likely been selected for during evolution to move large cargos. The comparable sizes of microtubule and pCBH ParM filaments indicate that larger filament architectures are not limited by the protomer fold. Instead, function appears to have been the evolutionary driving force to produce broad, complex filaments.

Original languageEnglish
Article number2856
JournalNature communications
Volume10
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

Fingerprint

Clostridium botulinum
Clostridium
Actin Cytoskeleton
Microtubules
Actins
filaments
Cryoelectron Microscopy
Protein Subunits
Cytoskeleton
Proteins
Plasmids
Microscopic examination
proteins
plasmids
polarity

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

The structure of a 15-stranded actin-like filament from Clostridium botulinum. / Koh, Fujiet; Narita, Akihiro; Lee, Lin Jie; Tanaka, Kotaro; Tan, Yong Zi; Dandey, Venkata P.; Popp, David; Robinson, Robert C.

In: Nature communications, Vol. 10, No. 1, 2856, 01.12.2019.

Research output: Contribution to journalArticle

Koh, Fujiet ; Narita, Akihiro ; Lee, Lin Jie ; Tanaka, Kotaro ; Tan, Yong Zi ; Dandey, Venkata P. ; Popp, David ; Robinson, Robert C. / The structure of a 15-stranded actin-like filament from Clostridium botulinum. In: Nature communications. 2019 ; Vol. 10, No. 1.
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