The structural evaluation of amyloid beta on lipid membranes

Satoka Aoyagi, Miki Iwamura, Toshinori Shimanouchi, Yuta Yokoyama, Hideo Iwai

Research output: Contribution to journalArticle

Abstract

Amyloid beta (Aβ) adsorption onto lipid membranes depending on the condition of a lipid was investigated by means of time-of-flight secondary ion mass spectrometry (ToF-SIMS). Aβ aggregation depending on the different hardness of lipid membrane has not been clarified yet although it is important to understand Alzheimer's disease. Aβ (1-40) on three different lipid membranes having different transition temperatures has been evaluated using ToF-SIMS in the previous study, and in this study, the differences between Aβ on liquid crystalline-phase lipid membranes and that on gel-phase lipid membranes were investigated in order to clarify the mechanisms of aggregation and peptide folding change. ToF-SIMS secondary ion images clearly showed Aβ distribution on lipid membranes. Because ToF-SIMS data is extremely complicated although it contains rich information, it was analysed by principal component analysis (PCA). Score images indicated by PCA are consistent with the images of secondary ions related to Aβ and are clearer than the secondary ion images. Moreover, PCA results suggest the difference between Aβ on different lipid membranes in terms of amino acid fragment ions, and the orientation of Aβ on 1,2-dipalmitoyl-sn-glycero-3-phosphocholine was indicated.

Original languageEnglish
JournalSurface and Interface Analysis
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Membrane Lipids
Amyloid
lipids
membranes
Secondary ion mass spectrometry
evaluation
secondary ion mass spectrometry
Principal component analysis
Ions
principal components analysis
Agglomeration
ions
Lipids
Peptides
Superconducting transition temperature
Amino acids
folding
peptides
Gels
amino acids

Keywords

  • Amyloid beta
  • Lipid
  • Structural analysis
  • ToF-SIMS

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

The structural evaluation of amyloid beta on lipid membranes. / Aoyagi, Satoka; Iwamura, Miki; Shimanouchi, Toshinori; Yokoyama, Yuta; Iwai, Hideo.

In: Surface and Interface Analysis, 2016.

Research output: Contribution to journalArticle

Aoyagi, Satoka ; Iwamura, Miki ; Shimanouchi, Toshinori ; Yokoyama, Yuta ; Iwai, Hideo. / The structural evaluation of amyloid beta on lipid membranes. In: Surface and Interface Analysis. 2016.
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