TY - JOUR
T1 - The strain-dependent constitutive expression of murine serum amyloid-P component is regulated at the transcriptional level
AU - Itoh, Yohjiro
AU - Takeuchi, Sakae
AU - Shigemoto, Kazuhiro
AU - Kubo, Sachiho
AU - Handa, Setsuko
AU - Ishikawa, Naoshi
AU - Maruyama, Naoki
N1 - Funding Information:
This study was performed through Japan Foundation for Aging and Health, and was supported in part by Sandoz Pharmaceuticals, Japan. The excellent editorial assistance of Ms. Phyllis Minick is gratefully acknowledged. We thank Ms. Yoshie Urano and Ms. Tatsuko Utsuki for their excellent technical assistances.
PY - 1992/7/15
Y1 - 1992/7/15
N2 - The strain-dependent expression of murine serum amyloid P-component (SAP) has been known to be linked to the Sap locus. We have quantified the SAP mRNA in several inbred strains including DBA/2 and C57BL/6 mice which represent high and low producers of SAP at resting state, respectively, and found that the mRNA levels correlated well with the amount of SAP protein. Interestingly, the SAP mRNA level of F1 mouse between DBA/2 and C57BL/6 was low and similar to that of C57BL/6. Primer extension and ribonuclease (RNAase) protection analyses demonstrated that a single type of transcript was generated from the SAP gene and that the cap sites were identical regardless mouse strains tested under unstimulated and stimulated (by lipopolysaccharide (LPS) or interleukin-6 (IL-6)) conditions. To investigate possible structural difference of the SAP gene including 5′ flanking region, we have cloned, sequenced and compared the SAP genes from DBA/2 and C57BL/6 mice. Sequence analyses revealed that the 5′ flanking regulatory regions, as well as the coding regions, were well-conserved between the two strains. These results demonstrate that the strain-dependent SAP expression occurs at the transcriptional level but seems to be affected by neither different type of the transcripts nor structural difference of the 5′ flanking and coding regions of the SAP gene. It was suggested that a possible transcription factor with suppressive activity, which is encoded by a gene linked to Sap, may be involved.
AB - The strain-dependent expression of murine serum amyloid P-component (SAP) has been known to be linked to the Sap locus. We have quantified the SAP mRNA in several inbred strains including DBA/2 and C57BL/6 mice which represent high and low producers of SAP at resting state, respectively, and found that the mRNA levels correlated well with the amount of SAP protein. Interestingly, the SAP mRNA level of F1 mouse between DBA/2 and C57BL/6 was low and similar to that of C57BL/6. Primer extension and ribonuclease (RNAase) protection analyses demonstrated that a single type of transcript was generated from the SAP gene and that the cap sites were identical regardless mouse strains tested under unstimulated and stimulated (by lipopolysaccharide (LPS) or interleukin-6 (IL-6)) conditions. To investigate possible structural difference of the SAP gene including 5′ flanking region, we have cloned, sequenced and compared the SAP genes from DBA/2 and C57BL/6 mice. Sequence analyses revealed that the 5′ flanking regulatory regions, as well as the coding regions, were well-conserved between the two strains. These results demonstrate that the strain-dependent SAP expression occurs at the transcriptional level but seems to be affected by neither different type of the transcripts nor structural difference of the 5′ flanking and coding regions of the SAP gene. It was suggested that a possible transcription factor with suppressive activity, which is encoded by a gene linked to Sap, may be involved.
KW - Acute phase reactant
KW - Liver specific expression
KW - Serum amyloid P-component (SAP)
KW - Strain-dependent expression
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U2 - 10.1016/0167-4781(92)90024-T
DO - 10.1016/0167-4781(92)90024-T
M3 - Article
C2 - 1627642
AN - SCOPUS:0026683714
VL - 1131
SP - 261
EP - 269
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 3
ER -