Abstract
Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.
| Original language | English |
|---|---|
| Pages (from-to) | 8593-8602 |
| Number of pages | 10 |
| Journal | Journal of Virology |
| Volume | 80 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - Sep 2006 |
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ASJC Scopus subject areas
- Immunology
Cite this
The spread of rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures. / Wei, Taiyun; Kikuchi, Akira; Moriyasu, Yusuke; Suzuki, Nobuhiro; Shimizu, Takumi; Hagiwara, Kyoji; Chen, Hongyan; Takahashi, Mami; Ichiki-Uehara, Tamaki; Omura, Toshihiro.
In: Journal of Virology, Vol. 80, No. 17, 09.2006, p. 8593-8602.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The spread of rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures
AU - Wei, Taiyun
AU - Kikuchi, Akira
AU - Moriyasu, Yusuke
AU - Suzuki, Nobuhiro
AU - Shimizu, Takumi
AU - Hagiwara, Kyoji
AU - Chen, Hongyan
AU - Takahashi, Mami
AU - Ichiki-Uehara, Tamaki
AU - Omura, Toshihiro
PY - 2006/9
Y1 - 2006/9
N2 - Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.
AB - Various cytopathological structures, known as inclusion bodies, are formed upon infection of cultured leafhopper cells by Rice dwarf virus, a member of the family Reoviridae. These structures include tubules of approximately 85 nm in diameter which are composed of the nonstructural viral protein Pns10 and contain viral particles. Such tubular structures were produced in heterologous non-host insect cells that expressed Pns10 of the virus. These tubules, when associated with actin-based filopodia, were able to protrude from the surface of cells and to penetrate neighboring cells. A binding assay in vitro revealed the specific binding of Pns10 to actin. Infection of clusters of cells was readily apparent 5 days after inoculation at a low multiplicity of infection with the virus, even in the presence of neutralizing antibodies. However, treatment of host cells with drugs that inhibited the elongation of actin filaments abolished the extension of Pns10 tubules from the surface of cells, with a significant simultaneous decrease in the extent of infection of neighboring cells. These results together revealed a previously undescribed aspect of the intercellular spread of Rice dwarf virus, wherein the virus exploits tubules composed of a nonstructural viral protein and actin-based filopodia to move into neighboring cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=33748676100&partnerID=8YFLogxK
U2 - 10.1128/JVI.00537-06
DO - 10.1128/JVI.00537-06
M3 - Article
C2 - 16912308
AN - SCOPUS:33748676100
VL - 80
SP - 8593
EP - 8602
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 17
ER -