The speckled epidermal nuclear immunofluorescence of mixed connective tissue disease seems to develop as an in vitro phenomenon

K. Iwatsuki, H. Tagami, S. Imaizumi, M. Ginoza, M. Yamada

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The pathogenesis of speckled epidermal nuclear immunofluorescence in patients with mixed connective tissue disease (MCTD) was studied by reproducing this reaction in guinea-pigs, using serum samples containing high titre antibody to ribonucleoprotein (RNP). Immunofluorescence studies on specimens obtained from guinea-pig skin into which serum samples containing high titre RNP antibody had been injected intradermally, revealed positive epidermal nuclear staining for IgG. This speckled immunofluorescence was demonstrable immediately after injection and remained so for 24 or 48 h. The pattern of fluorescence was similar in all cases, and there was no penetration of RNP antibody through the cell membrane. The epidermal nuclear fluorescence was not detected with sera at a dilution of 1:100 or more. These results provide strong evidence that the epidermal nuclear immunofluorescence observed in patients with high titre antibody to RNP develops as an in vitro phenomenon.

Original languageEnglish
Pages (from-to)653-657
Number of pages5
JournalBritish Journal of Dermatology
Volume107
Issue number6
DOIs
Publication statusPublished - 1982
Externally publishedYes

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Mixed Connective Tissue Disease
Ribonucleoproteins
Fluorescent Antibody Technique
Antibodies
Guinea Pigs
Fluorescence
Serum
Immunoglobulin G
Cell Membrane
Staining and Labeling
Skin
Injections
In Vitro Techniques

ASJC Scopus subject areas

  • Dermatology

Cite this

The speckled epidermal nuclear immunofluorescence of mixed connective tissue disease seems to develop as an in vitro phenomenon. / Iwatsuki, K.; Tagami, H.; Imaizumi, S.; Ginoza, M.; Yamada, M.

In: British Journal of Dermatology, Vol. 107, No. 6, 1982, p. 653-657.

Research output: Contribution to journalArticle

Iwatsuki, K. ; Tagami, H. ; Imaizumi, S. ; Ginoza, M. ; Yamada, M. / The speckled epidermal nuclear immunofluorescence of mixed connective tissue disease seems to develop as an in vitro phenomenon. In: British Journal of Dermatology. 1982 ; Vol. 107, No. 6. pp. 653-657.
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