The soybean sugar transporter gmSWEET15 mediates sucrose export from endosperm to early embryo

Shoudong Wang, Kengo Yokosho, Runze Guo, James Whelan, Yong Ling Ruan, Jian Feng Ma, Huixia Shou

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Soybean (Glycine max) seed is primarily composed of a mature embryo that provides a major source of protein and oil for humans and other animals. Early in development, the tiny embryos grow rapidly and acquire large quantities of sugars from the liquid endosperm of developing seeds. An insufficient supply of nutrients from the endosperm to the embryo results in severe seed abortion and yield reduction. Hence, an understanding of the molecular basis and regulation of assimilate partitioning involved in early embryo development is important for improving soybean seed yield and quality. Here, we used expression profiling analysis to show that two paralogous sugar transporter genes from the SWEET (Sugars Will Eventually be Exported Transporter) family, GmSWEET15a and GmSWEET15b, were highly expressed in developing soybean seeds. In situ hybridization and quantitative real-time PCR showed that both genes were mainly expressed in the endosperm at the cotyledon stage. GmSWEET15b showed both efflux and influx activities for sucrose in Xenopus oocytes. In Arabidopsis (Arabidopsis thaliana), knockout of three AtSWEET alleles is required to see a defective, but not lethal, embryo phenotype, whereas knockout of both GmSWEET15 genes in soybean caused retarded embryo development and endosperm persistence, resulting in severe seed abortion. In addition, the embryo sugar content of the soybean knockout mutants was greatly reduced. These results demonstrate that the plasma membrane sugar transporter, GmSWEET15, is essential for embryo development in soybean by mediating Suc export from the endosperm to the embryo early in seed development.

Original languageEnglish
Pages (from-to)2133-2141
Number of pages9
JournalPlant physiology
Volume180
Issue number4
DOIs
Publication statusPublished - Aug 1 2019

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Endosperm
Soybeans
embryo (plant)
endosperm
Sucrose
transporters
Seeds
Embryonic Structures
sucrose
soybeans
sugars
Embryonic Development
seed abortion
embryogenesis
seed yield
Arabidopsis
seeds
knockout mutants
genes
Genes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

The soybean sugar transporter gmSWEET15 mediates sucrose export from endosperm to early embryo. / Wang, Shoudong; Yokosho, Kengo; Guo, Runze; Whelan, James; Ruan, Yong Ling; Ma, Jian Feng; Shou, Huixia.

In: Plant physiology, Vol. 180, No. 4, 01.08.2019, p. 2133-2141.

Research output: Contribution to journalArticle

Wang, Shoudong ; Yokosho, Kengo ; Guo, Runze ; Whelan, James ; Ruan, Yong Ling ; Ma, Jian Feng ; Shou, Huixia. / The soybean sugar transporter gmSWEET15 mediates sucrose export from endosperm to early embryo. In: Plant physiology. 2019 ; Vol. 180, No. 4. pp. 2133-2141.
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