The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons

T. Yoshii, Hirotaka Sakamoto, M. Kawasaki, H. Ozawa, Y. Ueta, T. Onaka, K. Fukui, M. Kawata

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Vasopressin (AVP) plays an important role in anxiety-related and social behaviors. Single-prolonged stress (SPS) has been established as an animal acute severe stress model and has been shown to induce a lower adrenocorticotropic hormone (ACTH) response upon cortisol challenge. Here, we show results from immunoassays for AVP, ACTH, and corticosterone (CORT), and in situ hybridizations for AVP mRNA performed 7 days after SPS exposure. Immunofluorescence for AVP was also performed during the 7-day period following SPS exposure and after an additional forced swimming stress paradigm. We observed that the plasma concentrations of AVP, ACTH, and CORT were not altered by SPS; ACTH content in the pituitary and AVP mRNA expression in the supraoptic nucleus (SON) were significantly reduced by SPS. During the 7-day period following SPS, the intensity of immunoreactivity, the size of the soma, and the immunoreactive optical density of the dendrites of AVP neurons in the SON all increased. An apparent reduction in the intensity of AVP immunoreactivity was observed in the SON at 4 h after additional stress. Additional forced swimming led to a rapid increase in the dendritic AVP content only in the controls and not in the SPS-treated rats. These findings suggest that AVP is a potential biomarker for past exposure to severe stress and that alterations in AVP may affect the development of pathogenesis in stress-related disorders.

Original languageEnglish
Pages (from-to)466-474
Number of pages9
JournalNeuroscience
Volume156
Issue number3
DOIs
Publication statusPublished - Oct 15 2008
Externally publishedYes

Fingerprint

Vasopressins
Supraoptic Nucleus
Adrenocorticotropic Hormone
Neurons
Corticosterone
Messenger RNA
Social Behavior
Carisoprodol
Dendrites
Immunoassay
In Situ Hybridization
Fluorescent Antibody Technique
Hydrocortisone
Anxiety
Biomarkers

Keywords

  • paraventricular nucleus
  • post-traumatic stress disorder
  • single-prolonged stress
  • supraoptic nucleus
  • vasopressin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons. / Yoshii, T.; Sakamoto, Hirotaka; Kawasaki, M.; Ozawa, H.; Ueta, Y.; Onaka, T.; Fukui, K.; Kawata, M.

In: Neuroscience, Vol. 156, No. 3, 15.10.2008, p. 466-474.

Research output: Contribution to journalArticle

Yoshii, T. ; Sakamoto, Hirotaka ; Kawasaki, M. ; Ozawa, H. ; Ueta, Y. ; Onaka, T. ; Fukui, K. ; Kawata, M. / The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons. In: Neuroscience. 2008 ; Vol. 156, No. 3. pp. 466-474.
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