Abstract
Vasopressin (AVP) plays an important role in anxiety-related and social behaviors. Single-prolonged stress (SPS) has been established as an animal acute severe stress model and has been shown to induce a lower adrenocorticotropic hormone (ACTH) response upon cortisol challenge. Here, we show results from immunoassays for AVP, ACTH, and corticosterone (CORT), and in situ hybridizations for AVP mRNA performed 7 days after SPS exposure. Immunofluorescence for AVP was also performed during the 7-day period following SPS exposure and after an additional forced swimming stress paradigm. We observed that the plasma concentrations of AVP, ACTH, and CORT were not altered by SPS; ACTH content in the pituitary and AVP mRNA expression in the supraoptic nucleus (SON) were significantly reduced by SPS. During the 7-day period following SPS, the intensity of immunoreactivity, the size of the soma, and the immunoreactive optical density of the dendrites of AVP neurons in the SON all increased. An apparent reduction in the intensity of AVP immunoreactivity was observed in the SON at 4 h after additional stress. Additional forced swimming led to a rapid increase in the dendritic AVP content only in the controls and not in the SPS-treated rats. These findings suggest that AVP is a potential biomarker for past exposure to severe stress and that alterations in AVP may affect the development of pathogenesis in stress-related disorders.
| Original language | English |
|---|---|
| Pages (from-to) | 466-474 |
| Number of pages | 9 |
| Journal | Neuroscience |
| Volume | 156 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 15 2008 |
| Externally published | Yes |
Fingerprint
Keywords
- paraventricular nucleus
- post-traumatic stress disorder
- single-prolonged stress
- supraoptic nucleus
- vasopressin
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons. / Yoshii, T.; Sakamoto, Hirotaka; Kawasaki, M.; Ozawa, H.; Ueta, Y.; Onaka, T.; Fukui, K.; Kawata, M.
In: Neuroscience, Vol. 156, No. 3, 15.10.2008, p. 466-474.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The single-prolonged stress paradigm alters both the morphology and stress response of magnocellular vasopressin neurons
AU - Yoshii, T.
AU - Sakamoto, Hirotaka
AU - Kawasaki, M.
AU - Ozawa, H.
AU - Ueta, Y.
AU - Onaka, T.
AU - Fukui, K.
AU - Kawata, M.
PY - 2008/10/15
Y1 - 2008/10/15
N2 - Vasopressin (AVP) plays an important role in anxiety-related and social behaviors. Single-prolonged stress (SPS) has been established as an animal acute severe stress model and has been shown to induce a lower adrenocorticotropic hormone (ACTH) response upon cortisol challenge. Here, we show results from immunoassays for AVP, ACTH, and corticosterone (CORT), and in situ hybridizations for AVP mRNA performed 7 days after SPS exposure. Immunofluorescence for AVP was also performed during the 7-day period following SPS exposure and after an additional forced swimming stress paradigm. We observed that the plasma concentrations of AVP, ACTH, and CORT were not altered by SPS; ACTH content in the pituitary and AVP mRNA expression in the supraoptic nucleus (SON) were significantly reduced by SPS. During the 7-day period following SPS, the intensity of immunoreactivity, the size of the soma, and the immunoreactive optical density of the dendrites of AVP neurons in the SON all increased. An apparent reduction in the intensity of AVP immunoreactivity was observed in the SON at 4 h after additional stress. Additional forced swimming led to a rapid increase in the dendritic AVP content only in the controls and not in the SPS-treated rats. These findings suggest that AVP is a potential biomarker for past exposure to severe stress and that alterations in AVP may affect the development of pathogenesis in stress-related disorders.
AB - Vasopressin (AVP) plays an important role in anxiety-related and social behaviors. Single-prolonged stress (SPS) has been established as an animal acute severe stress model and has been shown to induce a lower adrenocorticotropic hormone (ACTH) response upon cortisol challenge. Here, we show results from immunoassays for AVP, ACTH, and corticosterone (CORT), and in situ hybridizations for AVP mRNA performed 7 days after SPS exposure. Immunofluorescence for AVP was also performed during the 7-day period following SPS exposure and after an additional forced swimming stress paradigm. We observed that the plasma concentrations of AVP, ACTH, and CORT were not altered by SPS; ACTH content in the pituitary and AVP mRNA expression in the supraoptic nucleus (SON) were significantly reduced by SPS. During the 7-day period following SPS, the intensity of immunoreactivity, the size of the soma, and the immunoreactive optical density of the dendrites of AVP neurons in the SON all increased. An apparent reduction in the intensity of AVP immunoreactivity was observed in the SON at 4 h after additional stress. Additional forced swimming led to a rapid increase in the dendritic AVP content only in the controls and not in the SPS-treated rats. These findings suggest that AVP is a potential biomarker for past exposure to severe stress and that alterations in AVP may affect the development of pathogenesis in stress-related disorders.
KW - paraventricular nucleus
KW - post-traumatic stress disorder
KW - single-prolonged stress
KW - supraoptic nucleus
KW - vasopressin
UR - http://www.scopus.com/inward/record.url?scp=53249155911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=53249155911&partnerID=8YFLogxK
U2 - 10.1016/j.neuroscience.2008.07.049
DO - 10.1016/j.neuroscience.2008.07.049
M3 - Article
C2 - 18723079
AN - SCOPUS:53249155911
VL - 156
SP - 466
EP - 474
JO - Neuroscience
JF - Neuroscience
SN - 0306-4522
IS - 3
ER -