Abstract
Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
| Original language | English |
|---|---|
| Pages (from-to) | 180-183 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 261 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 14 1983 |
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Keywords
- brain
- compound 48/80
- histamine
- HPLC
- liquid nitrogen freezing
- microwave irradiation
- tele-methylhistamine
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen : contamination by extraencephalic mast-cell histamine. / Oishi, Ryozo; Nishibori, Masahiro; Saeki, Kiyomi.
In: Brain Research, Vol. 261, No. 1, 14.02.1983, p. 180-183.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen
T2 - contamination by extraencephalic mast-cell histamine
AU - Oishi, Ryozo
AU - Nishibori, Masahiro
AU - Saeki, Kiyomi
PY - 1983/2/14
Y1 - 1983/2/14
N2 - Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
AB - Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
KW - brain
KW - compound 48/80
KW - histamine
KW - HPLC
KW - liquid nitrogen freezing
KW - microwave irradiation
KW - tele-methylhistamine
UR - http://www.scopus.com/inward/record.url?scp=0020656696&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020656696&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(83)91302-1
DO - 10.1016/0006-8993(83)91302-1
M3 - Article
C2 - 6839152
AN - SCOPUS:0020656696
VL - 261
SP - 180
EP - 183
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -