TY - JOUR
T1 - The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen
T2 - contamination by extraencephalic mast-cell histamine
AU - Oishi, Ryozo
AU - Nishibori, Masahiro
AU - Saeki, Kiyomi
PY - 1983/2/14
Y1 - 1983/2/14
N2 - Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
AB - Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
KW - HPLC
KW - brain
KW - compound 48/80
KW - histamine
KW - liquid nitrogen freezing
KW - microwave irradiation
KW - tele-methylhistamine
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U2 - 10.1016/0006-8993(83)91302-1
DO - 10.1016/0006-8993(83)91302-1
M3 - Article
C2 - 6839152
AN - SCOPUS:0020656696
VL - 261
SP - 180
EP - 183
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -