Abstract
Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.
| Original language | English |
|---|---|
| Pages (from-to) | 180-183 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 261 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 14 1983 |
Keywords
- HPLC
- brain
- compound 48/80
- histamine
- liquid nitrogen freezing
- microwave irradiation
- tele-methylhistamine
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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Cite this
Oishi, R., Nishibori, M., & Saeki, K. (1983). The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine. Brain Research, 261(1), 180-183. https://doi.org/10.1016/0006-8993(83)91302-1