The simultaneous assay of histamine and tele-methylhistamine in the rat brain after microwave irradiation and immersion in liquid nitrogen: contamination by extraencephalic mast-cell histamine

Ryozo Oishi, Masahiro Nishibori, Kiyomi Saeki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Histamine (HA) and tele-methylhistamine (t-MH) contents of the brain were assayed in the rats killed by various procedures. Microwave irradiation and freezing in liquid nitrogen caused a significant elevation of brain HA and we obtained evidence that the increased HA was from disintegrated mast cells outside of the brain. The method of near-freezing in liquid nitrogen proved to be most suitable for rapid cooling the brain when attempting to assay brain HA and t-MH.

Original languageEnglish
Pages (from-to)180-183
Number of pages4
JournalBrain Research
Volume261
Issue number1
DOIs
Publication statusPublished - Feb 14 1983

Keywords

  • HPLC
  • brain
  • compound 48/80
  • histamine
  • liquid nitrogen freezing
  • microwave irradiation
  • tele-methylhistamine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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