The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.)

Shan Guo Yao, Junko Mushika, Shin Taketa, Masahiko Ichii

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We previously reported a mutant, srt5, in which the short-root phenotype at the seedling stage could be partially rescued by exogenous abscisic acid (ABA) [Plant Sci. 163 (2002) 217]. In this paper, we describe that exogenously applied sucrose (Suc), glucose (Glu) or fructose (Fru), but not mannitol (Mtl) or glucose analogs, can also rescue root growth of the mutant to extents greater than ABA. Combined treatment reveals that root growth of the mutant under 1 μM ABA treatment can be further promoted by the addition of sugars. In contrast, application of ABA shows no promotive effects on 100 mM sugar-treated roots of srt5 seedlings. We also observed that endogenous sucrose contents in seeds and seedlings of srt5 are significantly lower, and starch breakdown in endosperm of srt5 is normal compared to wild-type. Furthermore, when treated with ABA, sucrose content in roots increases greatly in srt5, but decreases significantly in the wild-type. Collectively, these results suggest that energy deficiency is the cause of the srt5 phenotype, and ABA regulates root elongation of the mutant in a sugar-mediated way. The possible mechanism for ABA to promote root growth by enhancing the level of sugar in roots of srt5 is discussed.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalPlant Science
Volume167
Issue number1
DOIs
Publication statusPublished - Jul 2004
Externally publishedYes

Fingerprint

Abscisic Acid
Sugars
abscisic acid
root growth
Oryza sativa
sugars
mutation
rice
Mutation
Growth
Seedlings
Sucrose
mutants
sucrose
seedlings
energy deficiencies
Phenotype
phenotype
Glucose
Endosperm

Keywords

  • Abscisic acid
  • Rice
  • Root growth
  • srt5
  • Sugar

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.). / Yao, Shan Guo; Mushika, Junko; Taketa, Shin; Ichii, Masahiko.

In: Plant Science, Vol. 167, No. 1, 07.2004, p. 49-54.

Research output: Contribution to journalArticle

Yao, Shan Guo ; Mushika, Junko ; Taketa, Shin ; Ichii, Masahiko. / The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.). In: Plant Science. 2004 ; Vol. 167, No. 1. pp. 49-54.
@article{2cab18f0f93f44159dc34f99f4a9557b,
title = "The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.)",
abstract = "We previously reported a mutant, srt5, in which the short-root phenotype at the seedling stage could be partially rescued by exogenous abscisic acid (ABA) [Plant Sci. 163 (2002) 217]. In this paper, we describe that exogenously applied sucrose (Suc), glucose (Glu) or fructose (Fru), but not mannitol (Mtl) or glucose analogs, can also rescue root growth of the mutant to extents greater than ABA. Combined treatment reveals that root growth of the mutant under 1 μM ABA treatment can be further promoted by the addition of sugars. In contrast, application of ABA shows no promotive effects on 100 mM sugar-treated roots of srt5 seedlings. We also observed that endogenous sucrose contents in seeds and seedlings of srt5 are significantly lower, and starch breakdown in endosperm of srt5 is normal compared to wild-type. Furthermore, when treated with ABA, sucrose content in roots increases greatly in srt5, but decreases significantly in the wild-type. Collectively, these results suggest that energy deficiency is the cause of the srt5 phenotype, and ABA regulates root elongation of the mutant in a sugar-mediated way. The possible mechanism for ABA to promote root growth by enhancing the level of sugar in roots of srt5 is discussed.",
keywords = "Abscisic acid, Rice, Root growth, srt5, Sugar",
author = "Yao, {Shan Guo} and Junko Mushika and Shin Taketa and Masahiko Ichii",
year = "2004",
month = "7",
doi = "10.1016/j.plantsci.2004.02.025",
language = "English",
volume = "167",
pages = "49--54",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - The short-root mutation srt5 defines a sugar-mediated root growth in rice (Oryza sativa L.)

AU - Yao, Shan Guo

AU - Mushika, Junko

AU - Taketa, Shin

AU - Ichii, Masahiko

PY - 2004/7

Y1 - 2004/7

N2 - We previously reported a mutant, srt5, in which the short-root phenotype at the seedling stage could be partially rescued by exogenous abscisic acid (ABA) [Plant Sci. 163 (2002) 217]. In this paper, we describe that exogenously applied sucrose (Suc), glucose (Glu) or fructose (Fru), but not mannitol (Mtl) or glucose analogs, can also rescue root growth of the mutant to extents greater than ABA. Combined treatment reveals that root growth of the mutant under 1 μM ABA treatment can be further promoted by the addition of sugars. In contrast, application of ABA shows no promotive effects on 100 mM sugar-treated roots of srt5 seedlings. We also observed that endogenous sucrose contents in seeds and seedlings of srt5 are significantly lower, and starch breakdown in endosperm of srt5 is normal compared to wild-type. Furthermore, when treated with ABA, sucrose content in roots increases greatly in srt5, but decreases significantly in the wild-type. Collectively, these results suggest that energy deficiency is the cause of the srt5 phenotype, and ABA regulates root elongation of the mutant in a sugar-mediated way. The possible mechanism for ABA to promote root growth by enhancing the level of sugar in roots of srt5 is discussed.

AB - We previously reported a mutant, srt5, in which the short-root phenotype at the seedling stage could be partially rescued by exogenous abscisic acid (ABA) [Plant Sci. 163 (2002) 217]. In this paper, we describe that exogenously applied sucrose (Suc), glucose (Glu) or fructose (Fru), but not mannitol (Mtl) or glucose analogs, can also rescue root growth of the mutant to extents greater than ABA. Combined treatment reveals that root growth of the mutant under 1 μM ABA treatment can be further promoted by the addition of sugars. In contrast, application of ABA shows no promotive effects on 100 mM sugar-treated roots of srt5 seedlings. We also observed that endogenous sucrose contents in seeds and seedlings of srt5 are significantly lower, and starch breakdown in endosperm of srt5 is normal compared to wild-type. Furthermore, when treated with ABA, sucrose content in roots increases greatly in srt5, but decreases significantly in the wild-type. Collectively, these results suggest that energy deficiency is the cause of the srt5 phenotype, and ABA regulates root elongation of the mutant in a sugar-mediated way. The possible mechanism for ABA to promote root growth by enhancing the level of sugar in roots of srt5 is discussed.

KW - Abscisic acid

KW - Rice

KW - Root growth

KW - srt5

KW - Sugar

UR - http://www.scopus.com/inward/record.url?scp=2442450577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442450577&partnerID=8YFLogxK

U2 - 10.1016/j.plantsci.2004.02.025

DO - 10.1016/j.plantsci.2004.02.025

M3 - Article

VL - 167

SP - 49

EP - 54

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 1

ER -