Abstract
Area postrema neurons mediate various autonomic responses, including emesis. We examined the effects of propofol, a widely used anesthetic with antiemetic properties, on the hyperpolarization-activated cation current (I h) in rat area postrema neurons using a slice patch-clamp technique. Although propofol suppressed Ih of area postrema neurons in a dose-dependent manner that was similar to what we observed for the hippocampal CA1 neurons, the IC50 for Ih in area postrema neurons (38 μM) was more than six times less than that found for hippocampal CA1 neurons (235 μM). We conclude that rat area postrema neurons are exquisitely sensitive to propofol. Given that reductions of Ih are associated with decreased excitability in neurons, we believe that the known antiemetic effects of propofol anesthesia are at least partly a result of a direct action on area postrema neurons to lower their excitability.
| Original language | English |
|---|---|
| Pages (from-to) | 198-201 |
| Number of pages | 4 |
| Journal | Brain Research |
| Volume | 1015 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jul 23 2004 |
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Keywords
- Area postrema neuron
- CA1 neuron
- Excitable membranes and synaptic transmission
- Hyperpolarization-activated cation current
- Other ion channels
- Propofol
- Rat
- Slice
- Whole-cell patch-clamp
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
The sensitivity of hyperpolarization-activated cation current (I h) to propofol in rat area postrema neurons. / Funahashi, Makoto; Mitoh, Yoshihiro; Matsuo, Ryuji.
In: Brain Research, Vol. 1015, No. 1-2, 23.07.2004, p. 198-201.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The sensitivity of hyperpolarization-activated cation current (I h) to propofol in rat area postrema neurons
AU - Funahashi, Makoto
AU - Mitoh, Yoshihiro
AU - Matsuo, Ryuji
PY - 2004/7/23
Y1 - 2004/7/23
N2 - Area postrema neurons mediate various autonomic responses, including emesis. We examined the effects of propofol, a widely used anesthetic with antiemetic properties, on the hyperpolarization-activated cation current (I h) in rat area postrema neurons using a slice patch-clamp technique. Although propofol suppressed Ih of area postrema neurons in a dose-dependent manner that was similar to what we observed for the hippocampal CA1 neurons, the IC50 for Ih in area postrema neurons (38 μM) was more than six times less than that found for hippocampal CA1 neurons (235 μM). We conclude that rat area postrema neurons are exquisitely sensitive to propofol. Given that reductions of Ih are associated with decreased excitability in neurons, we believe that the known antiemetic effects of propofol anesthesia are at least partly a result of a direct action on area postrema neurons to lower their excitability.
AB - Area postrema neurons mediate various autonomic responses, including emesis. We examined the effects of propofol, a widely used anesthetic with antiemetic properties, on the hyperpolarization-activated cation current (I h) in rat area postrema neurons using a slice patch-clamp technique. Although propofol suppressed Ih of area postrema neurons in a dose-dependent manner that was similar to what we observed for the hippocampal CA1 neurons, the IC50 for Ih in area postrema neurons (38 μM) was more than six times less than that found for hippocampal CA1 neurons (235 μM). We conclude that rat area postrema neurons are exquisitely sensitive to propofol. Given that reductions of Ih are associated with decreased excitability in neurons, we believe that the known antiemetic effects of propofol anesthesia are at least partly a result of a direct action on area postrema neurons to lower their excitability.
KW - Area postrema neuron
KW - CA1 neuron
KW - Excitable membranes and synaptic transmission
KW - Hyperpolarization-activated cation current
KW - Other ion channels
KW - Propofol
KW - Rat
KW - Slice
KW - Whole-cell patch-clamp
UR - http://www.scopus.com/inward/record.url?scp=3042615422&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042615422&partnerID=8YFLogxK
U2 - 10.1016/j.brainres.2004.04.043
DO - 10.1016/j.brainres.2004.04.043
M3 - Article
C2 - 15223387
AN - SCOPUS:3042615422
VL - 1015
SP - 198
EP - 201
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1-2
ER -