The Salmonella FlgA protein, a putative periplasmic chaperone essential for flagellar P ring formation

Takayuki Nambu, Kazuhiro Kutsukake

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

P ring is a periplasmic substructure of the flagellar basal body and is believed to connect with the peptidoglycan layer in Salmonella. Two flagellar genes, flgA and flgl, are known to be indispensable for P ring formation. The flgl gene encodes the component protein of the P ring. However, the role of the flgA gene product in P ring assembly remained unknown. Here, evidence is presented that FlgA is synthesized as a precursor form and exported via the Sec secretory pathway into the periplasmic space where P ring formation takes place. Overproduction of the Flgl protein led flgA mutants to form flagella with a P ring, suggesting that FlgA plays an auxiliary role in P ring assembly. Far-Western blot analysis revealed that FlgA binds in vitro to both Flgl and FlgA itself. Though a direct Flgl-Flgl interaction in the absence of FlgA could not be demonstrated, an indirect or direct interaction between the Flgl proteins was observed in the presence of FlgA. FlgA alone was very unstable in vivo, but co-expression with Flgl could stabilize FlgA. This suggests the presence of FlgA-Flgl interaction in vivo. On the basis of these results, a hypothesis is proposed that FlgA acts as a periplasmic chaperone, which assists a polymerization reaction of Flgl into the P ring through FlgA-Flgl interaction.

Original languageEnglish
Pages (from-to)1171-1178
Number of pages8
JournalMicrobiology
Volume146
Issue number5
Publication statusPublished - May 2000
Externally publishedYes

Fingerprint

Far-Western Blotting
Basal Bodies
Gene Components
Periplasm
Proteins
Flagella
Peptidoglycan
Secretory Pathway
Salmonella
Polymerization
Genes
Salmonella FlgA protein
In Vitro Techniques

Keywords

  • Flagella
  • P ring
  • Periplasmic chaperone
  • Protein-protein interaction
  • Salmonella

ASJC Scopus subject areas

  • Microbiology

Cite this

The Salmonella FlgA protein, a putative periplasmic chaperone essential for flagellar P ring formation. / Nambu, Takayuki; Kutsukake, Kazuhiro.

In: Microbiology, Vol. 146, No. 5, 05.2000, p. 1171-1178.

Research output: Contribution to journalArticle

Nambu, Takayuki ; Kutsukake, Kazuhiro. / The Salmonella FlgA protein, a putative periplasmic chaperone essential for flagellar P ring formation. In: Microbiology. 2000 ; Vol. 146, No. 5. pp. 1171-1178.
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