The S-layer protein from Campylobacter rectus: Sequence determination and function of the recombinant protein

Manabu Miyamoto, Hiroshi Maeda, Michitaka Kitanaka, Susumu Kokeguchi, Shogo Takashiba, Yoji Murayama

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The gene encoding the crystalline surface layer (S-layer) protein from Campylobacter rectus, designated slp, was sequenced and the recombinant gene product was expressed in Escherichia coli. The gene consisted of 4086 nucleotides encoding a protein with 1361 amino acids. The N-terminal amino acid sequence revealed that Slp did not contain a signal sequence, but that the initial methionine residue was processed. The deduced amino acid sequence displayed some common characteristic features of S-layer proteins previously reported. A homology search showed a high similarity to the Campylobacter fetus S-layer proteins, especially in their N-terminus. The C-terminal third of Slp exhibited homology with the RTX toxins from Gram-negative bacteria via the region including the glycine-rich repeats. The Slp protein had the same N-terminal sequence as a 104-kDa cytotoxin isolated from the culture supernatants of C. rectus. However, neither native nor recombinant Slp showed cytotoxicity against HL-60 cells or human peripheral white blood cells. These data support the idea that the N-terminus acts as an anchor to the cell surface components and that the C-terminus is involved in the assembly and/or transport of the protein.

Original languageEnglish
Pages (from-to)275-281
Number of pages7
JournalFEMS Microbiology Letters
Volume166
Issue number2
Publication statusPublished - Sep 15 1998

Fingerprint

Campylobacter rectus
Recombinant Proteins
Sequence Analysis
Amino Acid Sequence
Campylobacter fetus
Genes
HL-60 Cells
Cytotoxins
Cellular Structures
Protein Sorting Signals
Gram-Negative Bacteria
Methionine
Glycine
Carrier Proteins
Proteins
Leukocytes
Nucleotides
Escherichia coli
Amino Acids
S-layer proteins

Keywords

  • Campylobacter rectus
  • DNA sequencing
  • RTX toxin
  • S-layer

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The S-layer protein from Campylobacter rectus : Sequence determination and function of the recombinant protein. / Miyamoto, Manabu; Maeda, Hiroshi; Kitanaka, Michitaka; Kokeguchi, Susumu; Takashiba, Shogo; Murayama, Yoji.

In: FEMS Microbiology Letters, Vol. 166, No. 2, 15.09.1998, p. 275-281.

Research output: Contribution to journalArticle

Miyamoto, Manabu ; Maeda, Hiroshi ; Kitanaka, Michitaka ; Kokeguchi, Susumu ; Takashiba, Shogo ; Murayama, Yoji. / The S-layer protein from Campylobacter rectus : Sequence determination and function of the recombinant protein. In: FEMS Microbiology Letters. 1998 ; Vol. 166, No. 2. pp. 275-281.
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