The roles of probiotic purple nonsulfur bacteria to control water quality and prevent acute hepatopancreatic necrosis disease (AHPND) for enhancement growth with higher survival in white shrimp (Litopenaeus vannamei) during cultivation

Supaporn Chumpol, Duangporn Kantachote, Teruhiko Nitoda, Hiroshi Kanzaki

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15 Citations (Scopus)

Abstract

This study aimed to investigate the potential of a mixed probiotic purple nonsulfur bacteria (PNSB) for controlling water quality and preventing acute hepatopancreatic necrosis disease (AHPND) to promote growth performance and increase survival of white shrimp (Litopenaeus vannamei) in postlarval stage during cultivation. Four probiotic PNSB (Rhodobacter sphaeroides strains SS15, S3W10, TKW17 and Afifella marina STW181) were investigated for controlling water quality using a mixed culture at a ratio of 1: 1 (roughly 1 × 108 cells mL− 1 for each, every week 1–7) as follows; T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) and found that throughout 8 weeks shrimp cultivation the most effective sets to reduce NH4 + and promote shrimp growth (based on weight and total length) were T1 and T3, respectively. Hence, PNSB strains (SS15, S3W10 and STW181) were used as a mixed culture (1: 1: 1, roughly 1 × 108 cells mL− 1 for each at weeks 1, 2 and 3) assessing their potential to prevent AHPND by challenge test on shrimp with a virulent strain AHPND-causing Vibrio parahaemolyticus SR2 (roughly 1 × 105 cells mL− 1 at day 15) during shrimp cultivation for 30 days. Inoculated PNSB sets (positive control: only PNSB inoculation, and treatment: both PNSB and SR2 inoculations) significantly decreased (P < 0.05) the levels of NH4 +, NO2 , NO3 and chemical oxygen demand (COD) with significantly higher (P < 0.05) of dissolved oxygen (DO) compared to native control (no inoculation) and challenge test (only SR2 inoculation). The maximal growth performance of shrimp was observed in the positive control; while no significant difference (P > 0.05) was observed for other sets. PNSB survived and colonized in intestinal shrimp tract to prevent AHPND by increasing 11% survival rate of infected shrimp by strain SR2. The correlation coefficient between PNSB population and shrimp survival showed a positive strong correlation, but a negative strong correlation between vibrios population and shrimp survival. Overall results proved that a mixed three probiotic PNSB are a good candidate for applying in white shrimp cultivation to maintain water quality and to protect shrimp diseases for promoting shrimp growth with higher survival rate.

Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalAquaculture
Volume473
DOIs
Publication statusPublished - Apr 20 2017

Fingerprint

Rhodospirillaceae
probiotics
Litopenaeus vannamei
necrosis
shrimp
water quality
bacterium
inoculation
mixed culture
survival rate
Rhodobacter sphaeroides
marina
Vibrio parahaemolyticus
Vibrio

Keywords

  • Acute hepatopancreatic necrosis disease
  • Probiotics
  • Purple nonsulfur bacteria
  • Shrimp cultivation
  • Vibrio parahaemolyticus
  • Water quality

ASJC Scopus subject areas

  • Aquatic Science

Cite this

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title = "The roles of probiotic purple nonsulfur bacteria to control water quality and prevent acute hepatopancreatic necrosis disease (AHPND) for enhancement growth with higher survival in white shrimp (Litopenaeus vannamei) during cultivation",
abstract = "This study aimed to investigate the potential of a mixed probiotic purple nonsulfur bacteria (PNSB) for controlling water quality and preventing acute hepatopancreatic necrosis disease (AHPND) to promote growth performance and increase survival of white shrimp (Litopenaeus vannamei) in postlarval stage during cultivation. Four probiotic PNSB (Rhodobacter sphaeroides strains SS15, S3W10, TKW17 and Afifella marina STW181) were investigated for controlling water quality using a mixed culture at a ratio of 1: 1 (roughly 1 × 108 cells mL− 1 for each, every week 1–7) as follows; T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) and found that throughout 8 weeks shrimp cultivation the most effective sets to reduce NH4 + and promote shrimp growth (based on weight and total length) were T1 and T3, respectively. Hence, PNSB strains (SS15, S3W10 and STW181) were used as a mixed culture (1: 1: 1, roughly 1 × 108 cells mL− 1 for each at weeks 1, 2 and 3) assessing their potential to prevent AHPND by challenge test on shrimp with a virulent strain AHPND-causing Vibrio parahaemolyticus SR2 (roughly 1 × 105 cells mL− 1 at day 15) during shrimp cultivation for 30 days. Inoculated PNSB sets (positive control: only PNSB inoculation, and treatment: both PNSB and SR2 inoculations) significantly decreased (P < 0.05) the levels of NH4 +, NO2 −, NO3 − and chemical oxygen demand (COD) with significantly higher (P < 0.05) of dissolved oxygen (DO) compared to native control (no inoculation) and challenge test (only SR2 inoculation). The maximal growth performance of shrimp was observed in the positive control; while no significant difference (P > 0.05) was observed for other sets. PNSB survived and colonized in intestinal shrimp tract to prevent AHPND by increasing 11{\%} survival rate of infected shrimp by strain SR2. The correlation coefficient between PNSB population and shrimp survival showed a positive strong correlation, but a negative strong correlation between vibrios population and shrimp survival. Overall results proved that a mixed three probiotic PNSB are a good candidate for applying in white shrimp cultivation to maintain water quality and to protect shrimp diseases for promoting shrimp growth with higher survival rate.",
keywords = "Acute hepatopancreatic necrosis disease, Probiotics, Purple nonsulfur bacteria, Shrimp cultivation, Vibrio parahaemolyticus, Water quality",
author = "Supaporn Chumpol and Duangporn Kantachote and Teruhiko Nitoda and Hiroshi Kanzaki",
year = "2017",
month = "4",
day = "20",
doi = "10.1016/j.aquaculture.2017.02.033",
language = "English",
volume = "473",
pages = "327--336",
journal = "Aquaculture",
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T1 - The roles of probiotic purple nonsulfur bacteria to control water quality and prevent acute hepatopancreatic necrosis disease (AHPND) for enhancement growth with higher survival in white shrimp (Litopenaeus vannamei) during cultivation

AU - Chumpol, Supaporn

AU - Kantachote, Duangporn

AU - Nitoda, Teruhiko

AU - Kanzaki, Hiroshi

PY - 2017/4/20

Y1 - 2017/4/20

N2 - This study aimed to investigate the potential of a mixed probiotic purple nonsulfur bacteria (PNSB) for controlling water quality and preventing acute hepatopancreatic necrosis disease (AHPND) to promote growth performance and increase survival of white shrimp (Litopenaeus vannamei) in postlarval stage during cultivation. Four probiotic PNSB (Rhodobacter sphaeroides strains SS15, S3W10, TKW17 and Afifella marina STW181) were investigated for controlling water quality using a mixed culture at a ratio of 1: 1 (roughly 1 × 108 cells mL− 1 for each, every week 1–7) as follows; T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) and found that throughout 8 weeks shrimp cultivation the most effective sets to reduce NH4 + and promote shrimp growth (based on weight and total length) were T1 and T3, respectively. Hence, PNSB strains (SS15, S3W10 and STW181) were used as a mixed culture (1: 1: 1, roughly 1 × 108 cells mL− 1 for each at weeks 1, 2 and 3) assessing their potential to prevent AHPND by challenge test on shrimp with a virulent strain AHPND-causing Vibrio parahaemolyticus SR2 (roughly 1 × 105 cells mL− 1 at day 15) during shrimp cultivation for 30 days. Inoculated PNSB sets (positive control: only PNSB inoculation, and treatment: both PNSB and SR2 inoculations) significantly decreased (P < 0.05) the levels of NH4 +, NO2 −, NO3 − and chemical oxygen demand (COD) with significantly higher (P < 0.05) of dissolved oxygen (DO) compared to native control (no inoculation) and challenge test (only SR2 inoculation). The maximal growth performance of shrimp was observed in the positive control; while no significant difference (P > 0.05) was observed for other sets. PNSB survived and colonized in intestinal shrimp tract to prevent AHPND by increasing 11% survival rate of infected shrimp by strain SR2. The correlation coefficient between PNSB population and shrimp survival showed a positive strong correlation, but a negative strong correlation between vibrios population and shrimp survival. Overall results proved that a mixed three probiotic PNSB are a good candidate for applying in white shrimp cultivation to maintain water quality and to protect shrimp diseases for promoting shrimp growth with higher survival rate.

AB - This study aimed to investigate the potential of a mixed probiotic purple nonsulfur bacteria (PNSB) for controlling water quality and preventing acute hepatopancreatic necrosis disease (AHPND) to promote growth performance and increase survival of white shrimp (Litopenaeus vannamei) in postlarval stage during cultivation. Four probiotic PNSB (Rhodobacter sphaeroides strains SS15, S3W10, TKW17 and Afifella marina STW181) were investigated for controlling water quality using a mixed culture at a ratio of 1: 1 (roughly 1 × 108 cells mL− 1 for each, every week 1–7) as follows; T1 (S3W10 + SS15), T2 (S3W10 + TKW17) and T3 (S3W10 + STW181) and found that throughout 8 weeks shrimp cultivation the most effective sets to reduce NH4 + and promote shrimp growth (based on weight and total length) were T1 and T3, respectively. Hence, PNSB strains (SS15, S3W10 and STW181) were used as a mixed culture (1: 1: 1, roughly 1 × 108 cells mL− 1 for each at weeks 1, 2 and 3) assessing their potential to prevent AHPND by challenge test on shrimp with a virulent strain AHPND-causing Vibrio parahaemolyticus SR2 (roughly 1 × 105 cells mL− 1 at day 15) during shrimp cultivation for 30 days. Inoculated PNSB sets (positive control: only PNSB inoculation, and treatment: both PNSB and SR2 inoculations) significantly decreased (P < 0.05) the levels of NH4 +, NO2 −, NO3 − and chemical oxygen demand (COD) with significantly higher (P < 0.05) of dissolved oxygen (DO) compared to native control (no inoculation) and challenge test (only SR2 inoculation). The maximal growth performance of shrimp was observed in the positive control; while no significant difference (P > 0.05) was observed for other sets. PNSB survived and colonized in intestinal shrimp tract to prevent AHPND by increasing 11% survival rate of infected shrimp by strain SR2. The correlation coefficient between PNSB population and shrimp survival showed a positive strong correlation, but a negative strong correlation between vibrios population and shrimp survival. Overall results proved that a mixed three probiotic PNSB are a good candidate for applying in white shrimp cultivation to maintain water quality and to protect shrimp diseases for promoting shrimp growth with higher survival rate.

KW - Acute hepatopancreatic necrosis disease

KW - Probiotics

KW - Purple nonsulfur bacteria

KW - Shrimp cultivation

KW - Vibrio parahaemolyticus

KW - Water quality

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JO - Aquaculture

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