The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo

Mahito Sato, Shinichiro Yoshimura, Rika Hirai, Ayako Goto, Masataka Kunii, Nur Atik, Takashi Sato, Ken Sato, Reiko Harada, Junko Shimada, Toshimitsu Hatabu, Hiroshi Yorifuji, Akihiro Harada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

VAMP7 or tetanus neurotoxin-insensitive vesicle- associated membrane protein (TI-VAMP) has been proposed to regulate apical transport in polarized epithelial cells, axonal transport in neurons and lysosomal exocytosis. To investigate the function of VAMP7 in vivo, we generated VAMP7 knockout mice. Here, we show that VAMP7 knockout mice are indistinguishable from control mice and display a similar localization of apical proteins in the kidney and small intestine and a similar localization of axonal proteins in the nervous system. Neurite outgrowth of cultured mutant hippocampal neurons was reduced in mutant neurons. However, lysosomal exocytosis was not affected in mutant fibroblasts. Our results show that VAMP7 is required in neurons to extend axons to the full extent. However, VAMP7 does not seem to be required for epithelial cell polarity and lysosomal exocytosis.

Original languageEnglish
Pages (from-to)1383-1393
Number of pages11
JournalTraffic
Volume12
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • Apical transport
  • Axonal outgrowth
  • Cell polarity
  • Knockout mouse
  • Lysosomal exocytosis
  • SNARE

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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