TY - JOUR
T1 - The role of the optic lobe in circadian locomotor rhythm generation in the cricket, Gryllus bimaculatus, with special reference to PDH-immunoreactive neurons
AU - Okamoto, Akiko
AU - Mori, Hisako
AU - Tomioka, Kenji
N1 - Funding Information:
We thank Dr K.R. Rao of the University of West Florida for providing anti-β-PDH antiserum. This study was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2001/7/15
Y1 - 2001/7/15
N2 - The role of optic lobes in rhythm generation was examined by a combination of surgical operation and immunohistochemistry using anti-β-pigment-dispersing hormone (PDH) antibody. In adult male crickets, Gryllus bimaculatus, one optic lobe was removed and the contralateral optic lobe was surgically lesioned to varying extent. Locomotor rhythms persisted in most crickets with the optic nerve severed or with the lamina region removed, while they were lost when the lamina medulla complex was removed. The rhythm was also lost in all animals with the distal part of the medulla removed. In all cases, fragments of the PDH-immunoreactive neurons (PDH-IRNs) remained undegenerated in the brain, and in the animals with the medulla area partially removed, the shape of the PDH-IRNs showed a rough similarity to that in intact animals, but the number of fibers projecting toward the cerebral lobe was increased. These results confirm our previous conclusion that the optic lobe is indispensable for the generation of locomotor rhythm. They also strongly suggest that the pacemaker resides in the medulla area or between the lamina and the medulla area, and that the PDH-IRNs in the medulla are not the pacemaker neurons:
AB - The role of optic lobes in rhythm generation was examined by a combination of surgical operation and immunohistochemistry using anti-β-pigment-dispersing hormone (PDH) antibody. In adult male crickets, Gryllus bimaculatus, one optic lobe was removed and the contralateral optic lobe was surgically lesioned to varying extent. Locomotor rhythms persisted in most crickets with the optic nerve severed or with the lamina region removed, while they were lost when the lamina medulla complex was removed. The rhythm was also lost in all animals with the distal part of the medulla removed. In all cases, fragments of the PDH-immunoreactive neurons (PDH-IRNs) remained undegenerated in the brain, and in the animals with the medulla area partially removed, the shape of the PDH-IRNs showed a rough similarity to that in intact animals, but the number of fibers projecting toward the cerebral lobe was increased. These results confirm our previous conclusion that the optic lobe is indispensable for the generation of locomotor rhythm. They also strongly suggest that the pacemaker resides in the medulla area or between the lamina and the medulla area, and that the PDH-IRNs in the medulla are not the pacemaker neurons:
KW - Circadian rhythms
KW - Locomotor rhythm
KW - Optic lobe
KW - Pigment dispersing hormone
UR - http://www.scopus.com/inward/record.url?scp=0035879436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035879436&partnerID=8YFLogxK
U2 - 10.1016/S0022-1910(01)00061-0
DO - 10.1016/S0022-1910(01)00061-0
M3 - Article
AN - SCOPUS:0035879436
VL - 47
SP - 889
EP - 895
JO - Journal of Insect Physiology
JF - Journal of Insect Physiology
SN - 0022-1910
IS - 8
ER -