The role of sonic hedgehog signaling in osteoclastogenesis and jaw bone destruction

Tsuyoshi Shimo, Kenichi Matsumoto, Kiyofumi Takabatake, Eriko Aoyama, Yuichiro Takebe, Soichiro Ibaragi, Tatsuo Okui, Naito Kurio, Hiroyuki Takada, Kyoichi Obata, Pai Pang, Masahiro Iwamoto, Hitoshi Nagatsuka, Akira Sasaki

Research output: Contribution to journalArticle

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Abstract

Sonic hedgehog (SHH) and its signaling have been identified in several human cancers, and increased levels of its expression appear to correlate with disease progression and metastasis. However, the role of SHH in bone destruction associated with oral squamous cell carcinomas is still unclear. In this study we analyzed SHH expression and the role played by SHH signaling in gingival carcinoma-induced jawbone destruction. From an analysis of surgically resected lower gingival squamous cell carcinoma mandible samples, we found that SHH was highly expressed in tumor cells that had invaded the bone matrix. On the other hand, the hedgehog receptor Patched and the signaling molecule Gli-2 were highly expressed in the osteoclasts and the progenitor cells. SHH stimulated osteoclast formation and pit formation in the presence of the receptor activator for nuclear factor-κB ligand (RANKL) in CD11b+ mouse bone marrow cells. SHH upregulated phosphorylation of ERK1/2 and p38 MAPK, NFATc1, tartrate-resistant acid phosphatase (TRAP), and Cathepsin K expression in RAW264.7 cells. Our results suggest that tumor-derived SHH stimulated the osteoclast formation and bone resorption in the tumor jawbone microenvironment.

Original languageEnglish
Article numbere0151731
JournalPLoS One
Volume11
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

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Hedgehogs
Erinaceidae
Jaw
jaws
Osteogenesis
Bone
bones
Bone and Bones
Tumors
osteoclasts
Cells
Cathepsin K
Osteoclasts
Phosphorylation
p38 Mitogen-Activated Protein Kinases
Acid Phosphatase
squamous cell carcinoma
neoplasms
Squamous Cell Carcinoma
cathepsin K

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The role of sonic hedgehog signaling in osteoclastogenesis and jaw bone destruction. / Shimo, Tsuyoshi; Matsumoto, Kenichi; Takabatake, Kiyofumi; Aoyama, Eriko; Takebe, Yuichiro; Ibaragi, Soichiro; Okui, Tatsuo; Kurio, Naito; Takada, Hiroyuki; Obata, Kyoichi; Pang, Pai; Iwamoto, Masahiro; Nagatsuka, Hitoshi; Sasaki, Akira.

In: PLoS One, Vol. 11, No. 3, e0151731, 01.03.2016.

Research output: Contribution to journalArticle

Shimo, Tsuyoshi ; Matsumoto, Kenichi ; Takabatake, Kiyofumi ; Aoyama, Eriko ; Takebe, Yuichiro ; Ibaragi, Soichiro ; Okui, Tatsuo ; Kurio, Naito ; Takada, Hiroyuki ; Obata, Kyoichi ; Pang, Pai ; Iwamoto, Masahiro ; Nagatsuka, Hitoshi ; Sasaki, Akira. / The role of sonic hedgehog signaling in osteoclastogenesis and jaw bone destruction. In: PLoS One. 2016 ; Vol. 11, No. 3.
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