The role of pituitary gonadotropins in gonadal sex differentiation in the protogynous Malabar grouper, Epinephelus malabaricus

Ryosuke Murata, Yasuhisa Kobayashi, Hirofumi Karimata, Kazuo Kishimoto, Motofumi Kimura, Akio Shimizu, Masaru Nakamura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The aim of this study was to clarify the roles of 2 gonadotropins (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH), on sex differentiation in the protogynous Malabar grouper, Epinephelus malabaricus. To do this, the mRNA expression patterns of GTH subunits (cga, fshb, and lhb) in the fish pituitary throughout gonadal sex differentiation were investigated. Real-time reverse transcriptase (RT)-PCR showed that cga and fshb were present in the undifferentiated and ovarian differentiation stages, and that the expression levels significantly increased after ovarian differentiation (AOD). However, lhb was not expressed before ovarian differentiation (BOD) and was first detected AOD. Next, to investigate the differentiation and distribution of Fshb and Lhb-producing cells in the pituitary of fish throughout gonadal sex differentiation, immunohistochemical analysis was used to detect teleost GTH subunits. Positive immunoreactivity against Fshb and Lhb was not detected in the pituitary BOD; Fshb and Lhb-positive cells first appeared in the pituitary AOD. It therefore seems unlikely that pituitary gonadotropins play a major role in the control of gonadal sex differentiation in the Malabar grouper.

Original languageEnglish
Pages (from-to)587-592
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume178
Issue number3
DOIs
Publication statusPublished - Sep 15 2012

Fingerprint

Epinephelus malabaricus
pituitary gonadotropins
Pituitary Gonadotropins
Sex Differentiation
grouper
sexual development
Fishes
follicle-stimulating hormone
Follicle Stimulating Hormone
gonadotropins
luteinizing hormone
Luteinizing Hormone
Reverse Transcriptase Polymerase Chain Reaction
Gonadotropins
fish
Real-Time Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
cells
Messenger RNA

Keywords

  • Follicle-stimulating hormone
  • Gonadotropins
  • Grouper
  • Luteinizing hormone
  • Pituitary
  • Sex differentiation

ASJC Scopus subject areas

  • Endocrinology

Cite this

The role of pituitary gonadotropins in gonadal sex differentiation in the protogynous Malabar grouper, Epinephelus malabaricus. / Murata, Ryosuke; Kobayashi, Yasuhisa; Karimata, Hirofumi; Kishimoto, Kazuo; Kimura, Motofumi; Shimizu, Akio; Nakamura, Masaru.

In: General and Comparative Endocrinology, Vol. 178, No. 3, 15.09.2012, p. 587-592.

Research output: Contribution to journalArticle

Murata, Ryosuke ; Kobayashi, Yasuhisa ; Karimata, Hirofumi ; Kishimoto, Kazuo ; Kimura, Motofumi ; Shimizu, Akio ; Nakamura, Masaru. / The role of pituitary gonadotropins in gonadal sex differentiation in the protogynous Malabar grouper, Epinephelus malabaricus. In: General and Comparative Endocrinology. 2012 ; Vol. 178, No. 3. pp. 587-592.
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