TY - JOUR
T1 - The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of collagen-induced arthritis in rats
AU - Ogata, Hiroomi
AU - Takeya, Motohiro
AU - Yoshimura, Teizo
AU - Takagi, Katsumasa
AU - Takahashi, Kiyoshi
PY - 1997/5/1
Y1 - 1997/5/1
N2 - Collagen-induced arthritis was produced in rats by intradermal immunization with type 11 collagen and the expression and production of monocyte chemoattractant protein-1 (MCP-1) were examined by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and Northern blot analysis. Two to three weeks after the immunization, the hindfeet showed swelling and redness, followed by the development of severe arthritis, particularly in the ankle joints. During this period, prominent infiltration of neutrophils and macrophages was observed. Sandwich ELISA and Northern blot analysis revealed that MCP-1 concentrations in the joint lavages and MCP-1 mRNA levels in the joint tissues both peaked at 2 weeks after the immunization. By immunohistochemistry, various types of cells, particularly neutrophils, macrophages, synovial cells, and vascular endothelial cells, stained positively for MCP-1. Finally, injection of a neutralizing monoclonal antibody against rat MCP-1 significantly decreased the number of exudate macrophages in the lesions and reduced the ankle swelling by about 30 per cent compared with controls. These results suggest that MCP-1 plays a critical role in this model in the recruitment of monocytes and in the development of arthritis.
AB - Collagen-induced arthritis was produced in rats by intradermal immunization with type 11 collagen and the expression and production of monocyte chemoattractant protein-1 (MCP-1) were examined by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and Northern blot analysis. Two to three weeks after the immunization, the hindfeet showed swelling and redness, followed by the development of severe arthritis, particularly in the ankle joints. During this period, prominent infiltration of neutrophils and macrophages was observed. Sandwich ELISA and Northern blot analysis revealed that MCP-1 concentrations in the joint lavages and MCP-1 mRNA levels in the joint tissues both peaked at 2 weeks after the immunization. By immunohistochemistry, various types of cells, particularly neutrophils, macrophages, synovial cells, and vascular endothelial cells, stained positively for MCP-1. Finally, injection of a neutralizing monoclonal antibody against rat MCP-1 significantly decreased the number of exudate macrophages in the lesions and reduced the ankle swelling by about 30 per cent compared with controls. These results suggest that MCP-1 plays a critical role in this model in the recruitment of monocytes and in the development of arthritis.
KW - arthritis
KW - macrophages
KW - monocyte chemoattractant protein
KW - monocytes
KW - pathogenesis
KW - rat model
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U2 - 10.1002/(SICI)1096-9896(199705)182:1<106::AID-PATH816>3.0.CO;2-A
DO - 10.1002/(SICI)1096-9896(199705)182:1<106::AID-PATH816>3.0.CO;2-A
M3 - Article
C2 - 9227349
AN - SCOPUS:0030909913
VL - 182
SP - 106
EP - 114
JO - Journal of Pathology
JF - Journal of Pathology
SN - 0022-3417
IS - 1
ER -