The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of collagen-induced arthritis in rats

Hiroomi Ogata, Motohiro Takeya, Teizo Yoshimura, Katsumasa Takagi, Kiyoshi Takahashi

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Collagen-induced arthritis was produced in rats by intradermal immunization with type 11 collagen and the expression and production of monocyte chemoattractant protein-1 (MCP-1) were examined by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and Northern blot analysis. Two to three weeks after the immunization, the hindfeet showed swelling and redness, followed by the development of severe arthritis, particularly in the ankle joints. During this period, prominent infiltration of neutrophils and macrophages was observed. Sandwich ELISA and Northern blot analysis revealed that MCP-1 concentrations in the joint lavages and MCP-1 mRNA levels in the joint tissues both peaked at 2 weeks after the immunization. By immunohistochemistry, various types of cells, particularly neutrophils, macrophages, synovial cells, and vascular endothelial cells, stained positively for MCP-1. Finally, injection of a neutralizing monoclonal antibody against rat MCP-1 significantly decreased the number of exudate macrophages in the lesions and reduced the ankle swelling by about 30 per cent compared with controls. These results suggest that MCP-1 plays a critical role in this model in the recruitment of monocytes and in the development of arthritis.

Original languageEnglish
Pages (from-to)106-114
Number of pages9
JournalJournal of Pathology
Volume182
Issue number1
DOIs
Publication statusPublished - May 1997
Externally publishedYes

Fingerprint

Experimental Arthritis
Chemokine CCL2
Immunization
Macrophages
Northern Blotting
Arthritis
Collagen Type XI
Joints
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Ankle Joint
Neutrophil Infiltration
Therapeutic Irrigation
Exudates and Transudates
Neutralizing Antibodies
Ankle
Monocytes
Neutrophils
Endothelial Cells
Monoclonal Antibodies

Keywords

  • arthritis
  • macrophages
  • monocyte chemoattractant protein
  • monocytes
  • pathogenesis
  • rat model

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of collagen-induced arthritis in rats. / Ogata, Hiroomi; Takeya, Motohiro; Yoshimura, Teizo; Takagi, Katsumasa; Takahashi, Kiyoshi.

In: Journal of Pathology, Vol. 182, No. 1, 05.1997, p. 106-114.

Research output: Contribution to journalArticle

Ogata, Hiroomi ; Takeya, Motohiro ; Yoshimura, Teizo ; Takagi, Katsumasa ; Takahashi, Kiyoshi. / The role of monocyte chemoattractant protein-1 (MCP-1) in the pathogenesis of collagen-induced arthritis in rats. In: Journal of Pathology. 1997 ; Vol. 182, No. 1. pp. 106-114.
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